GENETIC TOXICITY TESTING IN RODENTS

啮齿动物遗传毒性测试

• 批准号: 6356145
• 负责人: RAYMOND R TICE
• 金额: --
• 依托单位: INTEGRATED LABORATORY SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2001-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6356145
• 关键词: DNA damage; carcinogen testing; environmental toxicology; genetically modified animals; laboratory mouse; laboratory rat; mutagen testing; toxicant screening; toxicology information system

The objective of this project is the development of in vivo genetic toxicity data for the Environmental Toxicology Program (ETP) of the NTP. This work is achieved by in-house research programs and contract research, development, and testing programs. The contact obtained under this solicitation would allow for the testing of approximately 15 chemicals per year for 5 years for somatic cell genetic effects in rats and/or mice. Tests to be performed will include: chromosome aberrations (ABS) in bone marrow and peripheral lymphocytes; micronuclei (MN) in bone marrow, peripheral erythrocytes from sub-chronic studies (mice only), and other tissues (e.g., peripheral lymphocytes, lung, spleen); centromeric probe analysis of MN;single-cell gel electrophoresis assays of various tissues; and measurement of mutations in liver and other tissues of transgenic animals. Not all tests will be performed on all chemicals. The chemicals tested will be selected from among those that have undergone subchronic and chronic animal toxicity and/or carcinogenicity testing, or that are proposed for such testing, or are of special interest to the NTP. The results obtained from this contract will be used for setting priorities for long-term animal testing, and will provide information on mechanisms of action to support the interpretations of long-term animal toxicity results. Completion of these studies will also allow for the further development of the ETP's genetic toxicity database which will be used to determine the level of agreement between in vivo and in vitro genetic toxicity tests and the predictivity of in vivo results for rodent carcinogenesis.

该项目的目的是发展体内遗传 NTP的环境毒理学计划（ETP）的毒性数据。 这项工作是由内部研究计划和合同研究实现的 开发和测试计划。 在此获得的联系 征集将允许测试大约15种化学物质 在大鼠和/或小鼠中体细胞遗传作用的5年。 要执行的测试将包括：骨骼中的染色体畸变（ABS） 骨髓和外周淋巴细胞；骨髓中的微核（MN）， 亚基族研究（仅小鼠）和其他的外围红细胞和其他 组织（例如，外周淋巴细胞，肺，脾脏）；中心探针 分析Mn;各种组织的单细胞凝胶电泳测定； 和测量转基因的肝脏和其他组织中的突变 动物。 并非所有测试都将对所有化学物质进行。 测试的化学品将从经历的化学品中选择 亚基和慢性动物毒性和/或致癌性测试或 提议进行此类测试，或者是NTP特别感兴趣的。 从本合同获得的结果将用于设置 长期动物测试的优先级，并将提供有关 支持长期动物解释的作用机制 毒性结果。 这些研究的完成也将允许 ETP的遗传毒性数据库的进一步发展将是 用于确定体内和体外之间的一致性水平 遗传毒性测试和体内结果的预测性 致癌作用。