CORE--COMPUTER AND PERIPHERALS

核心——计算机及外设

• 批准号: 6448949
• 负责人: Stephen Morris Highstein
• 金额: $ 16.33万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6448949
• 关键词: biomedical facility; computer network; computers

In this project we will seek to resolve the origin of differences in tonic and phasic response output from the hair cell and cupula of the vestibular system. Specifically, we will determine if differences in encoded vestibular output is attributable to: (1) cupular motion and regional variation in hair cell stereociliary deflection; (2) afferents that are differentially (numerically and/or regionally) innervated by hair cells; and/or (3) that afferents may respond differentially to a transmitter quantum. This will be accomplished through a coordinated interdisciplinary effort encompassing digital video imaging image analysis, detailed electrophysiological recording and kinetic simulation studies described throughout this program project application. Silver's laboratory will focus upon the acquisition and analysis of images of stereociliary and cupular movements during head displacement, by developing a digital video light microscopic method for directly observation, analysis and relating movements of sub-cellular, cellular and supra-cellular structures in the vestibular end organ (VEO) related with vestibular function, using the toadfish (Opsanus tau) labyrinth as the model system, Reeves' group will focus upon advanced analytical imaging methods. High quality imagery of the performance of VEO components is needed to establish the structure- function relationships of these essential mechano-electrical transducers, especially in light of the advances made by the collaborating laboratories in hair cell electrophysiology and computational displacements of these transducers obtained on-line during an actual experiment is lacking. Recently, Silver, in collaboration with Highstein, accomplished direct video light microscopic imaging microscope capable of video observation and recording of stereociliary and cupular movements in situ in concert with concurrent electrophysiological recordings; (2) describe the sub-micrometer displacements of these structures during VEO movements via computational representations of image data (i.e., processed imagery, isosurfaces and isovolume) to aid in testing for the parity correspondence among actual VEO movements (this work), electrophysiological performance (Highstein and Boyle) and kinetic simulations (Rabbit, Miller). Integration of the information developed will facilitate assessment of normal and dysfunctional VEO performance in normal, and microgravity environments.

在这个项目中，我们将寻求解决差异的起源 来自毛孔和库布拉的补品和阶段性反应输出 前庭系统。 具体来说，我们将确定是否有差异 编码的前庭输出可归因于：（1）杯子运动和 毛细胞立体缩水的区域变化； （2）传统 由（数值和/或区域上）差异化的 毛细胞；和/或（3）传入可能对 发射器量子。 这将通过协调来实现 跨学科的努力包括数字视频成像图像 分析，详细的电生理记录和动力学模拟 整个计划项目应用程序中描述的研究。 银的 实验室将集中于对图像的获取和分析 头部位移期间的立体助理和持久运动 开发直接的数字视频光学微观方法 观察，分析和相关细胞，细胞的运动 前庭末端器官（VEO）中的超细胞结构相关 使用前庭功能，使用蟾蜍（opsanus tau）迷宫作为 Reeves的模型系统将重点放在高级分析上 成像方法。 VEO表现的高质量图像 需要组件来建立结构 - 功能关系 这些必需的机械电气传感器，尤其是在光下 在毛细细胞中合作实验室取得的进步 这些传感器的电生理学和计算位移 缺乏在实际实验中在线获得的。 最近， 银牌与Highstein合作完成了直接视频灯 微观成像显微镜，能够观察和记录 立体和杯状动作与并发一致 电生理记录； （2）描述亚微米 在VEO运动过程中，这些结构的位移通过计算 图像数据的表示（即处理的图像，等图和 isovolume）有助于测试实际之间的均等对应关系 VEO运动（这项工作），电生理性能（Highstein 和Boyle）和动力学模拟（Rabbit，Miller）。 整合 开发的信息将有助于评估正常和 在正常和微重力环境中的素食性能功能失调。