NEURAL MECHANISMS OF VESTIBULAR FUNCTION IN FISH

鱼类前庭功能的神经机制

• 批准号: 6448952
• 负责人: Stephen Morris Highstein
• 金额: $ 16.33万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6448952
• 关键词: Osteichthyes; afferent nerve; biological signal transduction; biomechanics; cilium /flagellum motility; confocal scanning microscopy; dyes; ear hair cell; electron microscopy; electronic stimulator; electrophysiology; endolymph; head movements; light microscopy; membrane potentials; morphology; neural information processing; perilymph; semicircular duct; sensory receptors; single cell analysis; vestibular nerve; vestibular pathway; vestibuloocular reflex; video microscopy; voltage /patch clamp

The overall goals of the proposal are to characterize the determinants of the response dynamics of the horizontal semicircular canal and to study the mechanisms and sequels of the efferent vestibular systems manipulation of these determinants. The response dynamics of the horizontal semicircular canal nerve have been documented in this species. There are three broad classes of afferent based upon their response dynamics across the frequency and amplitude spectrum studied. There are frequency and amplitude dependent non-linearities represented within the responses of certain classes of afferent. The contributions and significance of the putative differential motion of the center and periphery of the cupula and of hair cell receptor current frequency and amplitude dependent non-linearities to those in the primary afferents will be evaluated. Whole cell patch clamp techniques will be applied to hair cells isolated from defined functional regions of the crista to study the magnitude and kinetics of activation, inactivation, and deactivation of potassium currents. The current clamp responses to perturbing currents of these cells will be determined. Differential efferent action upon the various classes of primary afferent has also been documented. The implications and mechanisms of this differential action will be studied with morphological and physiological techniques. The amplitudes and shapes of miniature (m) EPSPs recorded from primary afferents will be measured before and during stimulation of efferents to assess the significance of the efferent evoked changes in EPSP characteristics to the output of the system. The activation sequelae of the efferent vestibular system upon horizontal canal afferents in free swimming fish will be studied utilizing multi-channel electrodes, under water acoustic telemetry and under water video. Thus, we hope to arrive at a general theory of efferent action in this species. Studies of the cellular and systems science aspects of the vestibular system and its efferent control add information about function and may bear upon possible future therapies and mechanisms for the control of motion sickness.

该提案的总体目标是表征决定因素 水平半圆形管的响应动力学和 研究传出前庭系统的机制和续集 操纵这些决定因素。响应动态 在该物种中已经记录了水平的半圆形神经。 根据他们的回应，有三个广泛的传入 研究了频率和振幅光谱的动力学。有 频率和幅度依赖性非线性表示 某些类别的传入的反应。贡献和 中心推定的差异运动的意义 库库拉和毛细胞受体电流频率和 主要传入中的振幅依赖性非线性 将进行评估。全细胞贴片夹技术将应用于 毛细胞从Crista的定义功能区域分离到 研究激活，灭活和动力学的大小和动力学 钾电流的失活。当前的夹具响应 这些细胞的扰动电流将被确定。微分 对各种类别的主要传入也有传出的行动 已记录。这种差异的含义和机制 将使用形态学和生理技术研究作用。 从初级记录的微型EPSP的幅度和形状 在刺激传出之前和期间，将测量传入者 评估EPSP诱发的诱发变化的重要性 系统输出的特性。激活后遗症 自由的水平运河传入的前庭系统 将使用多通道电极研究游泳鱼 水声遥测和水视频下。因此，我们希望到达 在该物种中传出作用的一般理论。研究 前庭系统的细胞和系统科学方面 传出控制添加有关功能的信息，并可能承受 可能的未来疗法和控制运动的机制 疾病。