NEURAL MECHANISMS OF VESTIBULAR FUNCTION IN FISH

鱼类前庭功能的神经机制

• 批准号: 6448952
• 负责人: Stephen Morris Highstein
• 金额: $ 16.33万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6448952
• 关键词: Osteichthyes; afferent nerve; biological signal transduction; biomechanics; cilium /flagellum motility; confocal scanning microscopy; dyes; ear hair cell; electron microscopy; electronic stimulator; electrophysiology; endolymph; head movements; light microscopy; membrane potentials; morphology; neural information processing; perilymph; semicircular duct; sensory receptors; single cell analysis; vestibular nerve; vestibular pathway; vestibuloocular reflex; video microscopy; voltage /patch clamp

The overall goals of the proposal are to characterize the determinants of the response dynamics of the horizontal semicircular canal and to study the mechanisms and sequels of the efferent vestibular systems manipulation of these determinants. The response dynamics of the horizontal semicircular canal nerve have been documented in this species. There are three broad classes of afferent based upon their response dynamics across the frequency and amplitude spectrum studied. There are frequency and amplitude dependent non-linearities represented within the responses of certain classes of afferent. The contributions and significance of the putative differential motion of the center and periphery of the cupula and of hair cell receptor current frequency and amplitude dependent non-linearities to those in the primary afferents will be evaluated. Whole cell patch clamp techniques will be applied to hair cells isolated from defined functional regions of the crista to study the magnitude and kinetics of activation, inactivation, and deactivation of potassium currents. The current clamp responses to perturbing currents of these cells will be determined. Differential efferent action upon the various classes of primary afferent has also been documented. The implications and mechanisms of this differential action will be studied with morphological and physiological techniques. The amplitudes and shapes of miniature (m) EPSPs recorded from primary afferents will be measured before and during stimulation of efferents to assess the significance of the efferent evoked changes in EPSP characteristics to the output of the system. The activation sequelae of the efferent vestibular system upon horizontal canal afferents in free swimming fish will be studied utilizing multi-channel electrodes, under water acoustic telemetry and under water video. Thus, we hope to arrive at a general theory of efferent action in this species. Studies of the cellular and systems science aspects of the vestibular system and its efferent control add information about function and may bear upon possible future therapies and mechanisms for the control of motion sickness.

该提案的总体目标是描述决定因素的特征 水平半规管的响应动力学和 研究传出前庭系统的机制和后果 操纵这些决定因素。的响应动力学 该物种有水平半规管神经。 根据反应的不同，传入信号可分为三大类 研究的频率和幅度谱上的动力学。有 频率和幅度相关的非线性表示在 某些类别传入的反应。贡献和 中心假定的微分运动的意义和 杯托周围和毛细胞受体的电流频率和 与初级传入神经振幅相关的非线性 将被评估。全细胞膜片钳技术将应用于 从嵴的特定功能区域中分离出的毛细胞 研究激活、失活和激活的幅度和动力学 钾电流失活。电流钳响应 这些细胞的扰动电流将被确定。微分 对各类初级传入神经的传出作用也 已被记录。这种差异的影响和机制 将通过形态学和生理学技术来研究行为。 从初级记录的微型（米）EPSP 的振幅和形状 在刺激传出神经之前和期间将测量传入神经 评估 EPSP 传出诱发变化的重要性 系统输出的特征。激活后遗症 自由水平管传入的传出前庭系统 将利用多通道电极研究游泳鱼， 水声遥测和水下视频。因此，我们希望能够到达 该物种传出作用的一般理论。研究的 前庭系统的细胞和系统科学方面及其 传出控制添加有关功能的信息，并可能影响 未来可能的运动控制疗法和机制 疾病。