CORE--NUCLEIC ACID FACILITY

核心--核酸设施

• 批准号: 6352707
• 负责人: MARSHA L. FRAZIER
• 金额: $ 25.41万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-18 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6352707
• 关键词: DNA; biomedical facility; cell line; genetic screening; genetic susceptibility; genetically modified animals; genotype; human genetic material tag; human tissue; laboratory mouse; neoplasm /cancer genetics; nucleic acid structure; oncogenes

The Nucleic Acid Core Facility is located in the Department of Gastrointestinal Oncology and Digestive Diseases in a 500 feet/2 laboratory. The Facility was created in the fall of 1996 through a CCSG supplement in Human Cancer Genetics. The NACF is a centralized facility for extraction of high-quality DNA from peripheral blood leukocytes and cell lines by using an Applied Biosystems Model 341 Nucleic Acid Extractor. This allows for the rapid isolation of highly purified, high- molecular-weight DNA. M.D. Anderson Cancer Center has a wealth of patient and family resources for studies of cancer-susceptibility genes. Most DNA isolation techniques used by individual laboratories required significant technical support and take 3 or more days. A centralized automated facility capable of producing PCR-grade DNA overnight from peripheral blood or cell is a major improvement in efficiency. The facility will be expanded to provide PCR-based genetic testing of transgenic animals. Because the high degree of product purity provided by the Model 341 Nucleic Acid Extractor will not be necessary for this testing, a more crude but rapid extraction procedure will be used to isolate DNA from mouse tails. The DNA will be subjected to PCR by using primates specifically designed to detect the transgenes, and animals positive for the transgenes will be identified. A large number of transgenic animal lines from over 20 different laboratories at M.D. Anderson are currently under investigation. Conducting DNA extraction from transgenic animal tails and PCR-based genetic testing in one centralized facility will be more efficient than performing these tasks in individual laboratories, as it will allow automation of each step by the use of Beckman Biomek 2000 robotic arms and multiplex PCR.

核酸核心设施位于 500英尺/2的胃肠道肿瘤学和消化疾病 实验室。该设施是在1996年秋天通过CCSG创建的 人类癌遗传学中的补充。 NACF是集中式设施 从外周血白细胞中提取高质量的DNA和 通过使用应用生物系统341核酸的细胞系 提取器。这允许快速隔离高度纯化的高 - 分子量体重DNA。 M.D. Anderson癌症中心有大量患者 以及用于研究癌症敏感性基因的家庭资源。大多数DNA 单个实验室使用的隔离技术需要大量 技术支持并花费3天或以上。集中自动化 能够从外围产生PCR级DNA过夜的设施 血液或细胞是效率的重大改善。设施将是 扩展以提供基于PCR的转基因动物的基因检测。 因为Model 341提供了高度的产品纯度 对于此测试，核酸提取器是不需要的，更多 粗糙但快速提取程序将用于隔离DNA 鼠标尾巴。 DNA将通过使用灵长类动物对PCR进行PCR 专门设计用于检测转基因的，动物呈阳性 转基因将被识别。大量转基因动物 M.D. Anderson的20多个不同实验室的线路目前正在 正在调查。从转基因动物中进行DNA提取 一个集中式设施中的尾巴和基于PCR的基因测试将是 比在单个实验室中执行这些任务更有效 它将通过使用Beckman Biomek 2000来自动化每个步骤 机器人臂和多重PCR。