CORE--MICROSCOPY AND MOLECULAR CYTOCHEMISTRY

核心——显微镜和分子细胞化学

• 批准号: 6269663
• 负责人: BILL R BRINKLEY
• 金额: $ 14.82万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6269663
• 关键词: biomedical facility; confocal scanning microscopy; electron microscopy; fluorescence microscopy; histochemistry /cytochemistry; image processing; in situ hybridization; microscopy; photography; tissue /cell preparation

The Microscopy and Molecular Cytochemistry Core (Core C) will provide instrumentation, expertise and service as related to microscopy, cytochemistry, immunocytochemistry, cytopathology, histochemistry and photography as needed by each of the four project components. This core will provide the following specific functions; (1) instrumentation and technical expertise on conventional bright field and epifluorescence microscopy, Laser Scanning Confocal Microscopy and image analysis, (2) hist and cytochemistry, immunocytochemistry, paraffin and frozen sections, special staining procedures, and in situ hybridization, (3) electron microscopy and, (4) photography including black and white or color prints, development of 35 mm film, darkroom procedures and photographic copying and macro-photography of dyeinjected mammary glands and whole animals. The core leader and personnel will be available to provide assistance and advice on experimental designs as related to any technique or procedure in microscopy and cytochemistry.

显微镜和分子细胞化学核心（核心 C）将提供 与显微镜相关的仪器、专业知识和服务， 细胞化学、免疫细胞化学、细胞病理学、组织化学和 四个项目组成部分所需的摄影。 这个核心 将提供以下具体功能； (1) 仪器仪表和 传统明场和落射荧光的技术专长 显微镜、激光扫描共焦显微镜和图像分析，(2) hist 以及细胞化学、免疫细胞化学、石蜡和冰冻切片， 特殊染色程序和原位杂交，（3）电子 显微镜检查，以及 (4) 摄影术，包括黑白或彩色照片， 开发 35 毫米胶片、暗室程序和照相复印 注射染料的乳腺和整个动物的宏观摄影。 这 核心领导和人员将提供帮助和 与任何技术或程序相关的实验设计的建议 显微镜和细胞化学。