ENRICHMENT ACTIVITIES

浓缩活动

• 批准号: 6204158
• 负责人: SHIVA P SINGH
• 金额: $ 8.62万
• 依托单位: ALABAMA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6204158
• 关键词: career; curriculum; health science profession; health science research; meeting /conference /symposium; postgraduate education; teacher; training; university student; workshop

The objectives of the proposed enrichment plan are: (1) to provide opportunities for minority science students to become acquainted with and to participate in biomedical research in order to motivate them to pursue graduate studies in the biomedical sciences, and (2) to provide opportunities for science faculty to upgrade their research skills and be involved in active sponsored research and student training. The proposed activities include: (a) faculty and student participation in seminars and workshops conducted by visiting scientists at Alabama State University campus, (b) faculty participation in workshops, extramural research, and "retooling" experiences at off-campus research institutions, (c) students' participation in extramural biomedical research during the summer at major universities in the United States, (d) students taking BIO 350, Introduction to Biomedical Research Techniques at Alabama State University, (e) faculty and student participation in Alabama State University's Biomedical Science Club activities and the College of Arts and Science's annual research symposium, and (f) attendance (by faculty and students) and presentation of research papers at national scientific meetings. We believe these activities will contribute to an improved intellectual and scientific environment in natural sciences which will motivate more science faculty to get actively involved in research, and more students to pursue graduate studies in biomedical sciences. Grant=S06GM082190006 Salmonella typhimurium is a gram-negative pathogen that primarily evokes gastroenteritis in man, but causes typhoid fever-like disease in mice. The outer membrane (OM) of S. typhimurium and other gram-negative bacteria contains lipopolysaccharide (LPS) and a family of major proteins called, porins. These OM components are the primary targets for immune recognition by the host during bacterial infections. This project will sequence the gene that encodes OmpD porin, and use monoclonal antibodies (MAbs) to determine the molecular specificity of the humoral immune response to infection by S. typhimurium. The specific aims of the project are to map the antigenic regions on the OmpD porin of Salmonella, to clone and sequence the ompD gene, to identify the dominant and immunoprotective porin and porin-LPS epitopes, and to determine the effect of LPS structure on the recognition of these epitopes during bacterial infections. The antigenic regions of OmpD will be mapped by Western immunoblot reactivity of cyanogen bromide-generated porin peptides with a panel of existing anti-OmpD MAbs. Identification of dominant (and immunoprotective) epitopes will involve the analysis of polyclonal immune sera by binding competition (enzyme-linked immunosorbent assay and Western blots) with a panel of existing anti- Salmonella MAbs. These MAbs are either specific for the surface epitopes of OmpD or OmpC porin, or for the porin-LPS complex, and they either prolong the survival of mice or confer significant protection against endotoxemia and/or bacteremia in experimental murine salmonellosis. The effect of LPS structure on the specificity of anti-porin and anti-LPS antibodies that arise during infection will be studied using smooth and rough variants of S. typhimurium. The long-term goal of this project is to elucidate the potential application of immunodominant porin epitopes for development of Salmonella-specific diagnostic probes and vaccines, and for insertion and expression of foreign genes. Since all gram- negative bacteria probably contain similar OM protein structures, the proposed research is relevant to gram-negative bacterial pathogenesis in general. The project is also designed to train minority students who will participate in all phases of this research project. Students will review literature, formulate research ideas, design and carry out experiments using modern biomedical research techniques, and gain experience in writing and presentation of scientific papers at regional and national meetings.

拟议的丰富计划的目标是：（1）提供 少数族裔科学专业的学生熟悉并 参加生物医学研究以激励他们追求 生物医学科学研究生研究，（2）提供 科学教师提升他们的研究技能和 参与积极赞助的研究和学生培训。 这 拟议的活动包括：（a）教师和学生参与 阿拉巴马州的访问科学家举办的研讨会和讲习班 大学校园，（b）教职员工参加研讨会，外壁外 研究，并在校外研究中“重新修改”经验 机构，（c）学生参与壁外生物医学 夏季在美国主要大学的研究， （d）参加Bio 350的学生，生物医学研究简介 阿拉巴马州立大学的技术（E）教职员工 参加阿拉巴马州立大学的生物医学科学俱乐部 活动与艺术与科学学院的年度研究 研讨会，（f）出席（由教师和学生）和演讲 在国家科学会议上的研究论文。 我们相信这些 活动将有助于改善的智力和科学 自然科学的环境将激发更多的科学教师 积极参与研究，更多的学生追求 生物医学科学研究生研究。 格兰特= S06GM082190006 鼠伤寒沙门氏菌是一种革兰氏阴性病原体，主要唤起 人类的胃肠炎，但在小鼠中引起伤寒疾病。 鼠伤寒的外膜（OM）和其他革兰氏阴性 细菌含有脂多糖（LPS）和主要蛋白质家族 叫，波林。 这些OM成分是免疫的主要目标 细菌感染期间宿主的识别。 这个项目将 序列编码OMPD Porin的基因并使用单克隆抗体 （mAb）确定体液免疫的分子特异性 鼠伤寒链球菌对感染的反应。 特定目标 项目将在沙门氏菌的OPMD孔林上绘制抗原区域， 克隆并序列OMPD基因，以确定主要和 免疫保护孔蛋白和孔蛋白-LPS表位，并确定 LPS结构对这些表位的识别期间的影响 细菌感染。 OMPD的抗原区域将由 氰基溴化生成的西洋的西方免疫印迹反应性 带有一组现有抗抗脏mab的肽。 识别 显性（和免疫保护）表位将涉及分析 通过结合竞争（酶连接）多克隆免疫血清 免疫吸附测定和蛋白质印迹）带有一组现有抗 沙门氏菌mabs。 这些mAb是特异性的 OPMD或OMPC Porin，或用于Porin-LPS复合物，它们要么 延长小鼠的生存或提供明显的保护 实验性鼠沙门氏菌病中的内毒素血症和/或菌血症。 这 LPS结构对抗胞蛋白和抗LPS的特异性的影响 感染期间出现的抗体将使用平滑和 鼠伤寒的粗糙变体。 这个项目的长期目标是 阐明免疫主导孔蛋白表位的潜在应用 为了开发沙门氏菌特异性诊断探针和疫苗， 以及插入和表达外源基因。 由于所有革兰氏 阴性细菌可能包含相似的OM蛋白结构， 提出的研究与革兰氏阴性细菌发病机理有关 一般的。 该项目还旨在培训少数民族学生 将参与该研究项目的所有阶段。 学生会 审查文献，制定研究思想，设计并执行 使用现代生物医学研究技术的实验，并获得 在区域的写作和演示方面的经验 和国家会议。