METHODS OF IONIZATION IN MASS SPECTROSCOPY

质谱中的电离方法

• 批准号: 6290498
• 负责人: SANFORD P MARKEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6290498
• 关键词: aminoacid metabolism; anandamide; biomarker; biomedical equipment development; brain metabolism; eicosanoid metabolism; electrospray ionization mass spectrometry; ion cyclotron resonance spectrometry; kynurenine; liquid chromatography; mass spectrometry; method development; nitric oxide; trace elements; tryptophan

Methods for the analysis of protein structures are being improved, applied and tested in both independent and collaborative research projects. Chromatographic systems, both on-line and gel-based are being tested with for the primary structure determination of indoleamine-2,3- dioxygenase as a model glycoprotein. Collaborative studies on the identification of post-translational modifications or unique gene products are in progress. Methods are also being devised and tested to improve the detectability of trace organic compounds using mass spectrometry. The development of new derivatization reagents and methods to impart improved electron capture properties for negative ionization mass spectrometric detection has been continued and applied to the analysis of glutamate in cell cultures. Liquid-chromatography - electrospray mass spectrometry methods continue to be developed for the quantitative analyses of 13C-labeled NAD and NADP as products of 13C- tryptophan metabolism. Fully 13C and 15N labeled NAD has been isolated from yeast for use as an analytical standard. Hydrophilic interaction chromatography has been successfully used for improved separations of nucleosides and NAD. An interface for coupling capillary zone electrophoresis and electrospray mass spectrometry has been designed to enable enhanced detection of peptides and mixtures isolated from cell extracts and tissues. - liquid chromatography, mass spectrometry, electrospray, capillary electrophoresis, derivatization, MALDI/TOF, protein structure

在独立和协作研究项目中，正在改进，应用和测试蛋白质结构的分析方法。在线和基于凝胶的色谱系统正在测试，以确定吲哚胺-2,3-二氧酶作为模型糖蛋白的主要结构。关于鉴定翻译后修饰或独特基因产品的协作研究正在进行中。也正在设计和测试方法，以改善使用质谱法的痕量有机化合物的可检测性。持续并应用于细胞培养物中谷氨酸分析的负电离质谱检测的新衍生试剂和方法的开发，以提高电离质谱检测的电子捕获特性。液态染色体 - 电喷雾质谱法继续开发用于对13C标记的NAD和NADP作为13c-色氨酸代谢的产物的定量分析。已将标记为NAD的13C和15N完全从酵母中分离出来，以用作分析标准。亲水相互作用色谱已成功用于改善核苷和NAD的分离。已经设计了用于耦合毛细管区电泳和电喷雾质谱的界面，以增强对从细胞提取物和组织中分离的肽和混合物的检测。 - 液相色谱，质谱，电喷雾，毛细管电泳，衍生物，MALDI/TOF，蛋白质结构