U MINN OFF SITE LOW VOLTAGE SEM STERECON DVMT: CELL & MICROORGANISM ANTIBODIES

U MINN 场外低压 SEM 立体 DVMT：电池

• 批准号: 6280721
• 负责人: STANLEY ERLANDSEN
• 金额: $ 0.08万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280721
• 关键词: Mammalia; bacteria; biomedical equipment development; biomedical resource; computers; human tissue; immunology; infection; inflammation; lymphatic system; microscopy; parasitism

(Supported in part by NIH GM 23484 and R01 GM 49530 to S. Erlandsen). Sterecon is being used at the University of Minnesota for 3-D measurements of immunolabelled cell-surface proteins imaged by high-resolution, low-temperature, low-voltage SEM. 3-D measurements of proteins on the 10nm scale agree with the expected size of the labeled molecules. Measurements have been made on the surfaces of human lymphocytes and bacteria. Sterecon is also being used to measure surface area and flagella length of various pathogenic protozoa. Additional applications in confocal LM and hospital X-ray tomography are anticipated. Dr. Leith modified Sterecon for more convenient use with SEM and confocal images. M. Marko visited UMN to install and test Sterecon, and Dr. Erlandsen visited Albany twice for training. A method for testing and debugging Sterecon applications at remote sites over the Internet was developed.

（部分由 NIH GM 23484 和 R01 GM 49530 至 S. 埃尔兰森）。 Sterecon 被明尼苏达大学用于 免疫标记细胞表面蛋白的 3-D 测量 高分辨率、低温、低电压SEM。 3-D 测量 10nm 尺度上的蛋白质与预期的大小一致 标记分子。 已在以下表面进行了测量 人类淋巴细胞和细菌。 Sterecon 也被用于 测量各种病原体的表面积和鞭毛长度 原生动物。 共焦 LM 和医院 X 射线中的其他应用 预计进行断层扫描。 Leith博士对Sterecon进行了更多修改 方便地与 SEM 和共焦图像一起使用。 M. Marko 访问 UMN 安装并测试 Sterecon，Erlandsen 博士两次访问奥尔巴尼 训练。 一种用于测试和调试 Sterecon 应用程序的方法 开发了通过互联网的远程站点。