DECIDUALIZATION IN THE BABOON

狒狒的蜕变

• 批准号: 6164933
• 负责人: Asgerally T. Fazleabas
• 金额: $ 27.13万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6164933
• 关键词: DNA footprinting; actins; baboons; cell cell interaction; cell differentiation; decidua; embryo implantation; extracellular matrix proteins; gel mobility shift assay; gene expression; genetic regulatory element; insulinlike growth factor; integrins; molecular cloning; phosphorylation; polymerase chain reaction; pregnancy; protein binding; transcription factor; trophoblast; western blottings

DESCRIPTION: (Adapted from applicant's abstract) Based on their previous studies and preliminary data, the investigators hypothesize that during the initial stage of stromal differentiation, alphaSMA expression is regulated by the interaction between stromal cell integrins with the secreted extracellular matrix proteins laminin and fibronectin, and that during the second phase, a trophoblast factor, whose signal is mediated by cAMP, induces IGFBP-1 expression in decidualizing stromal fibroblasts, which in turn can downregulate alphaSMA. To investigate these hypotheses, they propose to characterize the mechanisms by which the integrin/ECM interactions could potentially regulate alphaSMA expression in isolated baboon stromal fibroblasts (Aim 1), identify the transcription factors that regulate IGFBP-1 gene expression in baboon decidual cells (Aim 2), and develop an in vitro implantation model to define the role of the baboon trophoblast in regulating the decidualization process (Aim 3).

描述：（改编自申请人的摘要）基于他们之前的 研究和初步数据，研究人员假设 基质分化的初始阶段，αSMA表达为 通过基质细胞整合素与 分泌细胞外基质蛋白层粘连蛋白和纤连蛋白， 在第二阶段，滋养层因子，其信号被介导 通过 cAMP，诱导蜕膜基质中 IGFBP-1 的表达 成纤维细胞，进而可以下调 alphaSMA。调查 这些假设，他们提出描述机制 整合素/ECM 相互作用可能调节 alphaSMA 在分离的狒狒基质成纤维细胞中表达（目标 1），确定 调节狒狒 IGFBP-1 基因表达的转录因子 蜕膜细胞（目标 2），并开发体外植入模型 定义狒狒滋养层在调节 蜕膜化过程（目标 3）。