RHODOBACTER SPHAEROIDES IN C-TYPE CYTOCHROME ASSEMBLY

C 型细胞色素组装中的球状红细菌

• 批准号: 2857060
• 负责人: CARLOS RIOS-VELAZQUEZ
• 金额: $ 1.94万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2857060
• 关键词: Rhodospirillales; bacterial genetics; bacterial proteins; cytochrome c; hemoprotein biosynthesis; northern blottings; nucleic acid sequence; protein purification; southern blotting; stoichiometry; transcription factor; western blottings

The long term of this proposal is to identify the function of ccmF and ccmH gene products in the biosynthesis of c-type cytochromes in the photosynthetic bacterium Rhodobacter sphaeroides. Specifically, this proposal look forward to answer the following questions: (1) What are the properties of Rhodobacter sphaeroides ccmFH and (2) What are the roles of CcmF and CcmH. Biochemical and genetical approaches will be used to determine if ccmFH are co-transcribe and to generate the respective mutants. Specific cellular location, structure and possible association with other host factors will be achieved by using translational fusions and antibodies against each polypeptide. The specific step where each of these proteins act in c-type cytochromes in strains lacking each proteins. Finally, by using spheroplast and individual proteins required for c-type cytochrome biosynthesis, a CcmF and CcmH functional assay will be desing.

该提案的长期目标是确定 ccmF 的功能和 ccmH 基因产物在 c 型细胞色素的生物合成中 光合细菌球形红杆菌。具体来说，这 提案期待回答以下问题：（1）什么是 球形红杆菌 ccmFH 的特性和 (2) 的作用是什么 CcmF 和 CcmH。 将使用生化和遗传学方法来确定 ccmFH 是否 共同转录并产生各自的突变体。具体的 细胞位置、结构以及与其他宿主的可能关联 因素将通过使用翻译融合和抗体来实现 针对每个多肽。这些蛋白质中的每一个的具体步骤 在缺乏每种蛋白质的菌株中，在 c 型细胞色素中发挥作用。最后，由 使用原生质球和 c 型细胞色素所需的单个蛋白质 生物合成，将设计 CcmF 和 CcmH 功能测定。