FUNCTIONAL GENOMICS OF UNCULTIVATED SOIL BACTERIA

未耕种土壤细菌的功能基因组学

基本信息

批准号：
6385223
负责人：
MARK R LILES
金额：
$ 4.2万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-07-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/6385223
关键词：
Escherichia coli Rhodospirillales antibiotics bacteria bacterial genetics drug discovery /isolation functional /structural genomics genetic library green fluorescent proteins microorganism culture molecular cloning soil microbiology soil sampling transfection /expression vector

项目摘要

New vistas in the biological world have been revealed through the use of molecular techniques for assessing microbial diversity. Despite this revelation, whole division-level groups of bacteria exist in natural environments that are refractory to cultivation and potentially harbor previously undiscovered antibiotics. This study will access the functional diversity within the novel division Acidobacterium for the discovery of antibiotic agents. Using a collection of genomic clones derived from bacteria within this division, a heterologous expression system will be developed. By separating soil genomic DNA on the basis of G+C content, DNA fractions enriched for the presence of G+C content, DNA fractions enriched for the presence of Acidobacterium genomes will be used for genomic library construction in a broad host-range cosmid. Screening of this Acidobacterium-enriched genomic library for clones with activity against important human pathogens may result in the isolation of novel antibiotic agents, the genetic determinants and chemical structure of which will be determined.

通过使用分子技术评估微生物多样性，揭示了生物世界的新前景。尽管有这一发现，整个部门水平的细菌群仍然存在于自然环境中，这些环境难以培养，并且可能含有以前未发现的抗生素。这项研究将利用新部门酸杆菌内的功能多样性来发现抗生素。使用源自该部门内的细菌的基因组克隆集合，将开发异源表达系统。通过根据 G+C 含量分离土壤基因组 DNA、因 G+C 含量而富集的 DNA 级分、因酸杆菌基因组的存在而富集的 DNA 级分将用于在广泛的宿主范围粘粒中构建基因组文库。筛选这种富含酸杆菌的基因组文库，寻找具有抗重要人类病原体活性的克隆，可能会分离出新型抗生素药物，并确定其遗传决定因素和化学结构。