REGIMENTS OF OKA/MERCK VARICELLA VACCINE IN SERONEGATIVE HEALTHY CHILDREN

OKA/默克水痘疫苗用于血清阴性健康儿童的方案

• 批准号: 2784980
• 金额: $ 4.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2784980
• 关键词: adolescence (12-20); adult human (21+); age difference; attenuated microorganism; child (0-11); cytokine; enzyme linked immunosorbent assay; human therapy evaluation; immunity; leukocyte activation /transformation; live vaccine; longitudinal human study; varicella zoster virus; viral vaccines

Age-related differences in the persistence of immunity to varicella- zoster virus (VZV) were evaluated in 17 adults and 62 children previously immunized with various doses of live atenuated varicella vaccine. Immune response was evaluated by ELISA and VZV-specific lymphocyte stimulation assay. The presence of Th1 and Th2 type cytokines released by T lymphocytes stimulated with VZV was also evaluated. A one year post-vaccination, antibody seropositivity rates were 100% for adults and 94% for children; at five years post- vaccination seropositivity rates were 94% of adults and 95% of children. Cell-mediated immunity to VZV was maintained in 94% of adults and 81% of children at five years post-vaccination. Mean stimulation indices (SI) were significantly higher (p=0.04, Student's t test) at the persistence bleed compared to the one-year bleed in both adults and children. Cytokine responses (IL-2, IL-10 and INF-y) to VZV antigen were equivalent in adults and children. In conclusion, at five years post- vaccination, no differences in the persistence of immunity to VZV were observed in adults versus children as measured by the ELISA, VZV- specific lymphocyte stimulation assay and cytokine production.

与年龄相关的差异在免疫对水痘的持续性上存在 在17名成年人和62名儿童中评估了带状线病毒（VZV） 以前用各种剂量的活着的水素免疫 疫苗。 通过ELISA和VZV特异性评估免疫反应 淋巴细胞刺激测定法。 Th1和Th2类型的存在 通过VZV刺激的T淋巴细胞释放的细胞因子也是 评估。 疫苗接种后一年，抗体血清阳性率 成人为100％，儿童为94％；五年后 -​​ 疫苗接种血清阳性率为94％的成年人和95％的儿童。 在94％的成年人中维持了细胞介导的对VZV的免疫力，其中81％ 疫苗接种后五岁的孩子。 平均刺激指数（SI） 在持久性时，明显更高（P = 0.04，Student's t检验） 与成人和儿童的一年流血相比，流血。 细胞因子反应（IL-2，IL-10和INF-Y）对VZV抗原 等效于成人和儿童。 总之，五年后 疫苗接种，对VZV免疫的持续性没有差异为 通过ELISA测量的成年人与儿童观察到的VZV- 特定的淋巴细胞刺激测定法和细胞因子产生。