National COVID-19 Wastewater Epidemiology Surveillance Programme

国家 COVID-19 废水流行病学监测计划

• 批准号: NE/V010441/1
• 负责人: Andrew Singer
• 金额: $ 100.81万
• 依托单位: UK CENTRE FOR ECOLOGY & HYDROLOGY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FV010441%2F1
• 关键词: National; COVID; 19; Wastewater; Epidemiology

Wastewater-Based Epidemiology (WBE) requires relatively few resources compared to the systematic testing of populations. WBE is especially promising for novel infectious diseases, where asymptomatic cases might play a significant role in transmitting the virus. However, WBE is only now being used to monitor the spread of a pandemic infectious disease. Early studies by ourselves and others have shown that SARS-CoV-2 RNA can be recovered from wastewater, including from wastewater treatment plants (WWTP) preceding local COVID-19 hospitalisation activity. Given the challenge of making available diagnostic tests to the entire UK population, WBE represents a potentially low-cost and immediate mechanism for understanding levels of infection within large geographic areas.N-WESP aims to compare our methods with those of European & North American WBE teams in an inter-lab trial for understanding, supporting and improving the DEFRA COVID-19 measurements which will feed into the Joint Biosecurity Centre (JBC). We will also compare methods with DEFRA, the EA's and JBC whilst they explore options for finer geographical measurements. N-WESP will empower public health authorities with an optimised surveillance tool with maximal sensitivity and predictive power whose uncertainties have been well characterised.N-WESP will determine whether SARS-CoV-2 RNA in wastewater and sludge is infectious, and to what extent there might be downstream risks to human health. N-WESP will exploit catchment and, uniquely, sub-catchment-scale longitudinal surveillance to understand temporal and spatial heterogeneity, relationships to human disease burden distribution and whether there is potential outbreak 'hotspots' by surveilling sewer system nodes.

与人群的系统测试相比，基于废水的流行病学（WBE）需要相对较少的资源。 WBE对于新型的传染病特别有希望，在这种新型感染性疾病中，无症状病例可能在传播病毒中起重要作用。但是，WBE直到现在才被用于监测大流行传染病的传播。我们自己和其他人的早期研究表明，可以从废水中回收SARS-COV-2 RNA，包括从局部Covid-19-19-19，包括从废水处理厂（WWTP）中回收。考虑到对整个英国人口进行可用诊断测试的挑战，WBE代表了一种潜在的低成本和直接机制，可以理解大型地理区域内感染水平。N-WESP旨在将我们的方法与欧洲和北美WBE团队的方法进行比较。我们还将将方法与Defra，EA和JBC的方法进行比较，同时探索了更精细的地理测量的选择。 N-WESP将使用最大的敏感性和预测能力来使公共卫生机构具有优化的监视工具，其不确定性具有很好的特征。N-WESP将确定在废水和污泥中的SARS-COV-2 RNA是否具有感染性，并且在多大程度上对人类健康有多大风险。 N-WESP将利用集水区，并独特地，近距离尺度纵向监视，以了解时间和空间异质性，与人类疾病负担分布的关系以及是否有通过监视下水道系统节点进行监视的“热点”。

项目成果

Evaluation of variant calling algorithms for wastewater-based epidemiology using mixed populations of SARS-CoV-2 variants in synthetic and wastewater samples.

• DOI: 10.1099/mgen.0.000933
• 发表时间: 2023-04
• 期刊: MICROBIAL GENOMICS
• 影响因子: 3.9
• 作者: Bassano, Irene;Ramachandran, Vinoy K.;Khalifa, Mohammad S.;Lilley, Chris J.;Brown, Mathew R.;van Aerle, Ronny;Denise, Hubert;Rowe, William;George, Airey;Cairns, Edward;Wierzbicki, Claudia;Pickwell, Natalie D.;Carlile, Matthew;Holmes, Nadine;Payne, Alexander;Loose, Matthew;Burke, Terry A.;Paterson, Steve;Wade, Matthew J.;Grimsley, Jasmine M. S.
• 通讯作者: Grimsley, Jasmine M. S.

Population Infection Estimation from Wastewater Surveillance for SARS-CoV-2 in Nagpur, India During the Second Pandemic Wave

第二波大流行期间印度那格浦尔 SARS-CoV-2 废水监测中的人口感染估计

• DOI: 10.2139/ssrn.4349678
• 发表时间: 2023
• 作者: Acheampong E

City-wide wastewater genomic surveillance through the successive emergence of SARS-CoV-2 Alpha and Delta variants.

• DOI: 10.1016/j.watres.2022.119306
• 发表时间: 2022-11-01
• 期刊: WATER RESEARCH
• 影响因子: 12.8
• 作者: Brunner, F. S.;Brown, M. R.;Bassano, I;Denise, H.;Khalifa, M. S.;Wade, M. J.;van Aerle, R.;Kevill, J. L.;Jones, D. L.;Farkas, K.;Jeffries, A. R.;Cairns, E.;Wierzbicki, C.;Paterson, S.
• 通讯作者: Paterson, S.

Rapid Assessment of SARS-CoV-2 Variant-Associated Mutations in Wastewater Using Real-Time RT-PCR.

• DOI: 10.1128/spectrum.03177-22
• 发表时间: 2023-02-14
• 期刊: Microbiology spectrum
• 影响因子: 3.7

Occurrence of various viruses and recent evidence of SARS-CoV-2 in wastewater systems.

• DOI: 10.1016/j.jhazmat.2021.125439
• 发表时间: 2021-07-15
• 期刊: Journal of hazardous materials
• 影响因子: 13.6
• 作者: Ali W;Zhang H;Wang Z;Chang C;Javed A;Ali K;Du W;Niazi NK;Mao K;Yang Z
• 通讯作者: Yang Z