NSFDEB-NERC; Collaborative Resource; A phytochemical "tug-of-war" and its impact on organismal diversification and niche occupancy in Caryophyllales

NSFDEB-NERC；

• 批准号: NE/V003852/1
• 负责人: Samuel Brockington
• 金额: $ 30.93万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FV003852%2F1
• 关键词: NSFDEB; NERC; Collaborative; Resource; phytochemical

Our proposed work seeks to understand the step-wise evolution of complex specialized metabolic traits inflowering plants, and to explore how the evolution of such traits influence subsequent adaptation anddiversification in flowering plants. To address these questions, we will focus on the flowering plant orderCaryophyllales, which is well recognized for extraordinary adaptations to extreme environments andunusually high diversity of metabolites derived from the amino-acid Tyrosine. Our central hypothesis isthat changes in availability and abundance of Tyrosine in Caryophyllales has led to the evolution ofnumerous lineage-specific tyrosine-derived metabolites that in turn has profoundly influenced theadaptation and diversification of species within Caryophyllales. To test this hypothesis, we will build anevolutionary framework for Caryophyllales, that integrates transcriptomic, genomic, and metabolicdatasets, with patterns of trait evolution, on a macroevolutionary scale. Specifically, we will: 1) performan extensive survey to establish the occurrence and distribution of tyrosine-derived metabolic traits acrossCaryophyllales; 2) examine the association of these tyrosine-derived metabolic traits with organismaladaptation and diversification patterns; and 3) determine the evolutionary genetic mechanisms responsiblefor the biosynthesis of these metabolites. On completion of the proposed work, we expect to havecomprehensively described the extent of tyrosine-enriched metabolism in Caryophyllales, to have definedthe degree to which they are associated with organismal diversification patterns across Caryophyllales,and to have resolved the stepwise evolutionary assembly of the genetic pathways underlying complextyrosine-derived metabolic traits.Understanding the evolution of complex traits is a fundamental challenge for biologists, as the stepwisefashion by which such traits have evolved is not always readily apparent. Furthermore, the connectionsbetween the various stages of complex trait assembly (e.g. genetic, biochemical, and morphological) andsubsequent organismal diversification patterns are not well explored, with methodological approachesstill in their infancy. Recent advances in phylogenetics, with the integration of -omic scale data, nowprovide timely opportunities to marry the assembly of complex traits with lineage-specific and nichespecificorganismal diversification. Specialized metabolites are chemicals that confer adaptive advantagesin certain ecological and evolutionary contexts. The stepwise nature of the biosynthetic pathwaysunderlying complex specialized metabolites ensure that they are especially tractable for reconstructingstepwise evolution of complexity. While the phylogenetically restricted distributions of specializedmetabolites are fundamental to resolving the influence of complex traits on niche-specific and lineagespecificorganismal adaptation and diversification. Our approach, using the tyrosine-enriched specializedmetabolism in Caryophyllales as a model system, therefore has the potential to lead to new andfundamental insights into the causes and consequences of the evolutionary assembly of complex traits at avariety of evolutionary scales.

我们提出的工作旨在了解复杂的专业代谢性状流入植物的逐步演变，并探讨这种特征的演变如何影响开花植物中随后的适应和助剂。为了解决这些问题，我们将重点关注开花的植物有序磷脂，这是对极端环境的非凡适应，并且源自氨基酸酪氨酸的代谢产物的多样性。我们的中心假设是殖民地中酪氨酸的可用性和丰度的变化，导致了谱系特异性的酪氨酸衍生的代谢产物的进化，进而影响了毛藻类内物种的theaptation和物种多样化。为了检验这一假设，我们将为Caryophylales建立进化的框架，该框架将转录组，基因组和代谢数据集整合在一起，并在宏观进化量表上与性状进化模式。具体而言，我们将：1）执行广泛的调查，以建立跨千二氧化苯胺植物的酪氨酸衍生代谢性状的发生和分布； 2）检查这些酪氨酸衍生的代谢特征与生物阿拉底疗法和多样化模式的关联； 3）确定负责这些代谢产物生物合成的进化遗传机制。提议的工作完成后，我们期望在charyophylales中描述富含酪氨酸的代谢的程度对于生物学家来说，特征是一个基本挑战，因为这种特征发展的逐步时尚并不总是很明显。此外，复杂性状组装的各个阶段（例如遗传，生化和形态学）和苏子后期的生物多样化模式之间的连接均未得到充分的探索，而方法论学则在其婴儿期内进行方法学。系统发育学的最新进展，包括 - 摩西量表数据的整合，现在可以及时将复杂性状与谱系特异性和尼希比有生物多样化结合在一起。专门的代谢物是在某些生态和进化环境中赋予适应性优势的化学物质。生物合成途径的逐步性质在复杂的专用代谢物中逐步延伸，可确保它们对于重建复杂性的重建趋于趋势。尽管专用实体代谢物的系统发育限制分布是解决复杂性状对小众特异性和谱系特异性和谱系生物适应和多样化的影响的基础。因此，我们使用富含酪氨酸的专业量子代谢在石叶藻中作为模型系统，因此有可能导致对进化尺度贪婪的复杂性状进化组装进化组装的新原因和后果的新和基础见解。

项目成果

MycoRed: Betalain pigments enable in vivo real-time visualisation of arbuscular mycorrhizal colonisation.

• DOI: 10.1371/journal.pbio.3001326
• 发表时间: 2021-07
• 期刊: PLoS biology
• 影响因子: 9.8
• 作者: Timoneda A;Yunusov T;Quan C;Gavrin A;Brockington SF;Schornack S
• 通讯作者: Schornack S

A mycorrhiza-associated receptor-like kinase with an ancient origin in the green lineage.

• DOI: 10.1073/pnas.2105281118
• 发表时间: 2021-06-22
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Montero H;Lee T;Pucker B;Ferreras-Garrucho G;Oldroyd G;Brockington SF;Miyao A;Paszkowski U
• 通讯作者: Paszkowski U

Evolutionary blocks to anthocyanin accumulation and the loss of an anthocyanin carrier protein in betalain-pigmented Caryophyllales

• DOI: 10.1101/2022.10.19.512958
• 发表时间: 2022-10
• 期刊: bioRxiv
• 作者: Boas Pucker;Nathanael Walker-Hale;Won Yim;John Cushman;A. Crum;Ya Yang;Samuel F. Brockington