CORE--ASSAYS AND REAGENTS

核心——测定和试剂

• 批准号: 6108393
• 负责人: Alvin Rees Midgley
• 金额: $ 13.11万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6108393
• 关键词: antibody; biological products; biomedical facility; endocrinology; immunological substance; information systems; radioimmunoassay; radionuclides; radiopharmacology; reagent /indicator; reproduction

The overall goal of the Assays and Reagents Core is to provide state of the art assay methods, reagents, advice, training, assistance, and service in ways that will increase the cost effectiveness, quality, and timeliness of research performed by principal investigators of eligible projects. Specifically, the Core will: obtain, inventory, and maintain adequate stocks of analytical reagents needed by eligible projects (this includes developing, purchasing in bulk, requesting gifts or chemically synthesizing immunogens, first and second polyclonal and monoclonal antibodies and antibody fragments, standards, other reference and quality control preparations, and tracers labeled with radioiodide, biotin or enzymes); characterize and test acquired reagents and make them available to eligible projects; meet the needs of low volume Users, individuals with inadequate laboratory support, and Users with other needs by providing high quality, cost-effective service assays on a samples-in, results-out basis; incorporate advanced analytical quality control procedures in all activities; maintain performance records, inventories, and documentation for all reagents and assay systems provided by the Core; provide timely information, assistance, and advice in utilizing the methods effectively; develop or otherwise implement improved, state of the art assay methods and analytical procedures that reduce the use of radioactive isotopes and provide increased specificity, sensitivity, reliability and ease of use; provide quality control assessments for assays and assay kits provided to eligible projects and; teach assay technology and immunoassay data processing, and provide training and instruction in radioactive and hazardous materials safety, in universal precautions for biohazards, in laboratory safety and sanitation, and in applicable regulatory compliance.

检测和试剂核心的总体目标是提供 最先进的检测方法、试剂、建议、培训、帮助和 以提高成本效益、质量和效率的方式提供服务 合格的主要研究者进行研究的及时性 项目。具体来说，核心将：获取、库存和维护 符合条件的项目所需的充足的分析试剂库存（本 包括开发、批量采购、索要礼物或化学品 合成免疫原，第一和第二多克隆和单克隆 抗体和抗体片段、标准品、其他参考和质量 对照制剂和用放射性碘化物、生物素或标记的示踪剂 酶）；表征和测试获得的试剂并使其可用 符合资格的项目；满足小批量用户、个人的需求 实验室支持不足，以及有其他需求的用户 提供高质量、具有成本效益的样品检测服务， 结果得出的基础；纳入先进的分析质量控制 所有活动的程序；维护绩效记录、库存、 以及由该机构提供的所有试剂和测定系统的文档 核;提供及时的信息、帮助和建议，以利用 有效的方法；制定或以其他方式实施改进的状态 减少使用的先进测定方法和分析程序 放射性同位素并提供更高的特异性、灵敏度、 可靠性和易用性；提供质量控制评估 向符合条件的项目提供检测和检测试剂盒；教学分析 技术和免疫分析数据处理，并提供培训和 放射性和危险材料安全的普遍指导 生物危害、实验室安全和卫生以及 适用的监管合规性。