Rapid in-situ phytoplankton monitoring to support marine aquaculture and long term climate science

快速原位浮游植物监测以支持海水养殖和长期气候科学

• 批准号: NE/T008571/1
• 负责人: Keith Davidson
• 金额: $ 26.17万
• 依托单位: Scottish Association For Marine Science
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FT008571%2F1
• 关键词: Rapid; situ; phytoplankton; monitoring; support

Phytoplankton form the base of the marine food web. Through their primary production they are crucial to carbon cycling. Some species that form "harmful algal blooms (HABs) are harmful to human health and/or finfish and shellfish aquaculture operations.There are many thousands of species of phytoplankton. The diversity in responses of these to changing environmental conditions means that long term time series are required to understand the role of environment/climate in driving the productivity and biodiversity of our oceans. Phytoplankton increases (blooms) can occur over short timescales (days). Should blooming species be harmful, rapid early warning is required for the aquaculture industry and its regulators to protect human health (from HAB generated toxins that are vectored to humans by shellfish) and minimise mortalities, as a consequence of other HAB genera, of farmed fish.At present, the time and cost to analyse phytoplankton samples by microscopy or molecular methods prevents the high resolution monitoring required to a) understand climate effects and b) provide rapid early warning to the aquaculture industry and its regulators of HAB events. A solution to this problem is the Imaging FlowCytobot (IFCB) https://mclanelabs.com/imaging-flowcytobot/ This is an in-situ automated submersible imaging flow cytometer that also generates images of phytoplankton in-flow.The IFCB allow real time (every 20 minutes) monitoring of phytoplankton including HAB events. Once the instrument is "trained", images can be automatically classified to genus or even species level with accuracy comparable to that of human experts. Data are therefore comparable in quality with traditional microscope counts but are obtained at a much higher temporal resolution. Data collection is achieved through a novel combination of flow cytometric and video technology to capture high resolution images of suspended particles. Laser induced fluorescence and light scattering from individual particles are measured and used to trigger targeted image acquisition; the optical and image data are then transmitted to shore in real time. Images collected during this continuous monitoring are processed with automated image classification software.The IFCB can be deployed from a raft or pier to track the progression of annual phytoplankton cycles or HAB events. It can also be configured to sample from shipboard underway seawater systems, allowing phytoplankton communities to be monitored continuously along cruise routes.IFCBs have been used to collect data in a range of environments ranging from Florida to the Arctic with, e.g., the first harmful bloom so the shellfish biotoxin producing dinoflagellate Dinophysis being revealed by use of an IFCB (Campbell et al. 2010 J Phycol 46:60-75). IFCBs are now also being utilised for harmful algal bloom detection in Scandinavia and New Zealand.At present, there is no IFCB or similar capability in the UK. This instrument will therefore provide a step change in the capability of UK environmental science to monitor phytoplankton and provide early warning of the HABs that impact aquaculture.In this project we propose to purchase, train and deploy at IFCB in UK coastal waters allowing, for the first time, the production of the long term, temporally resolved data sets we require to understand the environmental control of phytoplankton and HABs in UK waters. The instrument will ordinarily be located at the Marine Scotland Coastal Observatory in Shetland close (a major site of aquaculture). IFCB data will be made freely available in real time via our web portal (www.HABreports.org) in a similar manner to an instrument in the Gulf of Mexico http://toast.tamu.edu/IFCB111. Our own expert data interpretation will enhance its benefit for aquaculture practitioners. The IFCB will therefore provide a long term data resource for scientists, policy makers the aquaculture industry and its regulators.

浮游植物构成了海洋食品网的基础。通过其主要生产，它们对于碳循环至关重要。一些形成“有害藻华（HAB）的物种对人类健康和/或鳍鱼和贝类水产养殖作战有害。有成千上万种浮游植物。这些对不断变化的环境条件的反应多样性意味着长期时间序列序列序列序列序列需要了解环境/气候在驱动我们海洋的生产力和生物多样性中的作用。它的调节剂保护人类健康（免受贝类通过贝类向人类载体的毒素产生的毒素），并由于其他HAB属而最大程度地减少死亡率，养殖鱼类，在场时，通过显微镜或分子分析浮游植物样品的时间和成本方法阻止了a）了解气候效应所需的高分辨率监控，b）向水产养殖业及其HAB事件的调节器提供快速的预警。解决此问题的一种解决方案是成像流胞机（IFCB）https://mclanelabs.com/imaging-flowcytobot/这是一种自动化自动化的肥大的想象流式细胞仪，还会生成phytoplankton in-flow.ife file file file file file file file flog。 （每20分钟）监测包括HAB事件在内的浮游植物。一旦“训练”仪器，就可以将图像自动分类到属甚至物种水平，其准确性与人类专家的准确性相当。因此，数据的质量与传统显微镜计数相当，但以更高的时间分辨率获得。数据收集是通过流式细胞仪和视频技术的新型组合来实现的，以捕获悬浮颗粒的高分辨率图像。测量激光诱导的荧光和来自单个颗粒的光散射，并用于触发目标图像采集；然后，将光学数据和图像数据实时传输到岸边。在此连续监视过程中收集的图像通过自动图像分类软件进行处理。可以从木筏或码头部署IFCB，以跟踪年度浮游植物周期或HAB事件的进展。它也可以配置为从船上的海水系统中进行采样，允许沿着巡航路线连续监视浮游植物社区。IFCB已用于在从佛罗里达到北极到北极的各种环境中收集数据，例如第一个有害的盛开因此，通过使用IFCB，贝类生物毒素产生了鞭毛鞭毛酸性的Dinophysy（Campbell等，2010 J Phycol 46：60-75）。现在，IFCB也被用于斯堪的纳维亚和新西兰的有害藻华检测。目前，英国没有IFCB或类似能力。因此，该工具将在英国环境科学监测浮游植物的能力上进行逐步改变，并提供影响水产养殖的HAB的预警。在本项目中，我们建议在英国沿海水域购买，培训和部署，以便在英国的IFCB上进行培训和部署第一次，我们需要的长期生产，暂时解决的数据集，我们需要了解英国水域中浮游植物和哈布斯的环境控制。该乐器通常位于设得兰群岛关闭的海洋苏格兰海岸天文台（水产养殖的主要地点）。 IFCB数据将通过我们的Web门户网站（www.habreports.org）实时免费提供，其方式与墨西哥湾http://toast.tamu.edu/ifcb111的仪器类似。我们自己的专家数据解释将增强其对水产养殖从业者的好处。因此，国际金融公司将为科学家，水产养殖行业及其监管机构提供长期数据资源。

项目成果

Editorial: Current challenges in providing early warning of harmful algal and microbiological risk to aquaculture

社论：当前在对水产养殖有害藻类和微生物风险提供预警方面面临的挑战

• DOI: 10.3389/fmars.2022.973925
• 发表时间: 2022
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: Neira Del Río P

Novel Methodologies for Providing In Situ Data to HAB Early Warning Systems in the European Atlantic Area: The PRIMROSE Experience

为欧洲大西洋地区 HAB 早期预警系统提供现场数据的新方法：PRIMROSE 经验

• DOI: 10.3389/fmars.2022.791329
• 发表时间: 2022
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: Ruiz-Villarreal M

Shelf Sea Biogeochemistry: Nutrient and carbon cycling in a temperate shelf sea water column

陆架海洋生物地球化学：温带陆架海水柱中的营养物和碳循环

• DOI: 10.1016/j.pocean.2019.102182
• 发表时间: 2019
• 期刊: Progress in Oceanography
• 影响因子: 4.1
• 作者: Sharples J

GlobalHAB. Evaluating, Reducing and Mitigating the Cost of Harmful Algal Blooms: A Compendium of Case Studies

全球HAB。

• 发表时间: 2020
• 作者: Davidson K

Current Status of Forecasting Toxic Harmful Algae for the North-East Atlantic Shellfish Aquaculture Industry

• DOI: 10.3389/fmars.2021.666583
• 发表时间: 2021-06-10
• 期刊: FRONTIERS IN MARINE SCIENCE
• 影响因子: 3.7
• 作者: Fernandes-Salvador, Jose A.;Davidson, Keith;Silke, Joe
• 通讯作者: Silke, Joe