CORE--FLOW CYTOMETRY

核心——流式细胞术

• 批准号: 6268010
• 负责人: SUSAN JACKSON
• 金额: $ 13.86万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6268010
• 关键词: AIDS; biomedical facility; cell biology; cell cycle; flow cytometry; immunofluorescence technique; light scattering; phagocytosis; transfection

This proposal is a request for continued partial funding of the Flow Cytometry Core Facility (FACS Core) within the UAB AIDS Center. The primary purposes of the FACS Core are to make accessible to AIDS Center investigators the equipment, trained personnel, and expertise to perform cell analysis and purification with state-of-the-art equipment and techniques on a reasonable cost for services basis, and to offer these services in a facility capable of handling potentially infectious material. The FACS Core serves over 30 individual investigators per year, with over 85% of actual booked time being used by AIDS Center members. At the present time, the core contains a FACStar single laster four parameter, two color cytometer/sorter. In the near future, using funds obtained from the UAB Health Services Foundation, this instrument will be replaced with a FACStar/plus six parameter, dual laser cytometer/sorter. The FACS Core and the Flow Cytometry Core Facility of the Multipurpose Arthritis & Musculoskeletal Diseases Center are operated jointly, an arrangement which results in improved service to investigators through increased flexibility in equipment and personnel. The two core facilities jointly employ two individuals who are both experienced cytometer operators. Funds are requested to ensure stable support for 50% of the operators' salaries, continuing education for the operators, and the cytometer maintenance contract. The requested level of support will be sufficient to cover slightly more than 50% of the actual cost of operating the FACS Core. This will allow the present rate of $50/hour for AIDS Center investigators to be sustained into the foreseeable future.

该提议是向流量进行部分资金的要求 UAB AIDS中心内的细胞仪核心设施（FACS核心）。这 FACS核心的主要目的是使艾滋病中心访问 调查人员的设备，训练有素的人员和专业知识 用最先进的设备进行细胞分析和净化 以合理的服务为基础的技术，并提供这些技术 能够处理潜在感染力的设施中的服务 材料。 FACS核心每年为30多个个人调查人员提供服务， 艾滋病中心成员使用了超过85％的实际预订时间。在 当前的时间，核心包含一个Facstar单层次四个 参数，两个彩色细胞仪/分类器。在不久的将来，使用资金 从UAB卫生服务基金会获得的该工具将是 由FACSTAR/PLUS六个参数取代，双激光细胞仪/分散器。 FACS核心和多功能的流式细胞仪核心设施 关节炎和肌肉骨骼疾病中心共同运作 安排通过 设备和人员的灵活性提高。两个核心设施 共同雇用两个经验丰富的细胞仪的人 操作员。要求资金确保对50％的稳定支持 运营商的薪水，对运营商的继续教育以及 细胞仪维护合同。请求的支持水平将是 足以覆盖实际运营成本的50％以上 FACS核心。这将允许艾滋病的目前每小时50美元 中心调查人员将在可预见的未来维持。