OLD YELLOW ENZYME--CLONING AND STRUCTURE

老黄酶--克隆和结构

• 批准号: 6244642
• 负责人: VINCENT MASSEY
• 金额: $ 2.22万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-20 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6244642
• 关键词: NAD(P)H dehydrogenase; Saccharomyces; aminoacid oxidase; computer assisted sequence analysis; enzyme mechanism; enzyme structure; fungal genetics; gene mutation; isozymes; molecular cloning; oxygenases

We have been using the GCG package maintained by the General Clinical Research Center in the study of Old Yellow Enzyme (OYE). Three isozymes of OYE from Saccharomyces have been cloned and sequenced. These sequences have a high homology, but the physiological function of OYE is still unknown. We continue to look for enzymes with sequence homology in an attempt to identify function. The crystal structure of one of the isozymes of OYE has been solved and we have been mutating significant residues to determine the effect on ligand binding and NADPH oxidation by the enzyme. We plan to use several of the features of the GCG program to design primers for additional mutation. The general focus of the lab is the study of riboflavin-containing enzymes. We are currently working on the cloning and characterization of two of these enzymes, D-Amino Acid Oxidase and 2-Methyl-3-Hydroxypyridine-5- Carboxylic Acid Oxygenase. In these studies we will continue to use the features of the GCG package.

我们一直在使用一般临床维护的GCG软件包 旧黄酶（OYE）研究的研究中心。 三个同工酶 来自糖疗法的Oye的Oye已被克隆并测序。 这些 序列具有很高的同源性，但是OYE的生理功能 仍然未知。 我们继续寻找序列的酶 同源性试图识别功能。 晶体结构 OYE的同工酶之一已解决，我们一直在突变 确定对配体结合和NADPH的影响的重要残留物 酶氧化。 我们计划使用的几个功能 GCG计划设计引物以进行附加突变。 将军 实验室的重点是含核黄素的酶的研究。 我们是 目前正在研究其中两个的克隆和表征 酶，D-氨基酸氧化酶和2-甲基-3-羟基吡啶-5-- 羧酸氧酶。 在这些研究中，我们将继续使用 GCG软件包的功能。