CORE--SCIENTIFIC

核心--科学

• 批准号: 6243845
• 负责人: WISE YOUNG
• 金额: $ 19万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-20 至 1998-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6243845
• 关键词: atomic absorption spectrometry; biomedical equipment development; biomedical facility; histology; histopathology; immunocytochemistry; microelectrodes; sectioning

The core provides five essential services for the Program: Microelectrode Fabrication Facility. We propose to consolidate and establish a state-of-the-art microelectrode fabrication facility that would be used by all investigators in the Center. The faculty of the Center posses substantial expertise in the fabrication and use of ion-selective microelectrodes and carbon fiber microelectrodes. Minimal equipment and supplies are requested, as well as a half-time technician to staff this facility to manufacture and calibrate the electrodes. Histological Facility: The Core will provide basic histological tissue sectioning and staining for all the projects. This includes frozen section, paraffin-section, and plastic sections for light microscopy and routine stains of the sections. Specialized stains, however, will be carried out by the individual projects. A half-time technician is requested for this purpose. Tissue Analysis Facility. The Core will provide atomic absorption analysis of tissue ions for all the projects. The ions that can be analyzed include Na, K, Ca, Mg, Fe, and others if necessary. In addition, the facility will do wet weight and dry weight measurements. Administrative Core. The Core will centralize orders for supplies and equipment to save money, effort, and time of individual investigators and technicians. Funds are requested for a half-time effort by the current laboratory manager and half-time effort of an administrative secretary. External Advisory Committee. A committee of five leading scientists in the field have been formed to advise and criticize the work of the center. These include Professor Harold Kimelberg, Ph.D of the State University of New York at Albany (glial cells and head injury), Professor Kevin Strange, Ph.D. Director of Critical Care Research laboratories at Harvard University (physiology and molecular biology of osmoregulation), professor Chung Hsu, M.D., Ph.D of the Department of Neurology at Washington University at St. Louis (ischemia and pharmacology of neuroprotection), Professor Andrew Blight of the Department of Neurosurgery at the University of North Carolina (spinal cord injury and morphological analysis of injured tissues, and Professor Theodore Holford, Ph.D., MPH of the Dept. of Epidemiology and Public Health at Yale University (statistics). In addition, individual projects have advisors and collaborators from NYU Medical Center, particularly James Salzer, M.D., Ph.D. (myelination and glial cultures) and Chiye, Ph.D. (histology and immunohistology). The External Advisory Committee will site-visit the Center once a year. Dr. Holford will be the statistical consultant for the Center.

核心为该计划提供了五个基本服务： 微电极制造设施。 我们建议合并和 建立最先进的微电极制造设施 中心的所有调查人员都使用。 中心教师 在离子选择性的制造和使用方面具有丰富的专业知识 微电极和碳纤维微电极。 最小设备和 请求供应，以及半个时间的技术人员来人员 制造和校准电极的设施。 组织学设施：核心将提供基本的组织学组织 所有项目的切片和染色。 这包括冷冻 截面，石蜡截面和塑料截面，用于光学显微镜和 各节的常规污渍。 但是，专门的污渍将是 由各个项目执行。 半场技术人员是 为此目的要求。 组织分析设施。 核心将提供原子吸收分析 所有项目的组织离子。 可以分析的离子包括 如有必要，NA，K，CA，MG，FE等。 此外，设施将 进行湿重和干重测量。 行政核心。 核心将集中供应订单和 节省个人调查人员的资金，精力和时间的设备 技术人员。 当前要求资金进行半场努力 行政秘书的实验室经理和半场努力。 外部咨询委员会。 一个由五个主要科学家组成的委员会 现场成立是为了建议和批评该中心的工作。 其中包括州立大学的Harold Kimelberg教授 纽约在奥尔巴尼（神经胶质细胞和头部受伤），凯文·斯特兰奇教授， 博士哈佛大学重症监护研究实验室主任 （渗透调节的生理学和分子生物学），钟教授， 华盛顿大学神经病学系的博士学位 路易斯（神经保护的缺血和药理学），安德鲁教授 北大学神经外科系的疫病 卡罗来纳州（脊髓损伤和受伤组织的形态分析， 以及西奥多·霍尔福德（Theodore Holford）教授，博士，流行病学系的MPH和 耶鲁大学的公共卫生（统计）。 另外，个人 项目有纽约大学医疗中心的顾问和合作者 特别是詹姆斯·萨尔泽（James Salzer），医学博士（髓鞘和神经胶质文化）和 Chiye博士（组织学和免疫组织学）。 外部咨询 委员会将每年进行一次访问该中心。 Holford博士将是 该中心的统计顾问。