CORE--PERIPHERAL STEM CELL LABORATORY

核心--外周干细胞实验室

• 批准号: 6239214
• 负责人: Leonard Stanley Sender
• 金额: $ 13万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-10 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239214
• 关键词: biomedical facility; blood treatment; flow cytometry; hematopoietic stem cells; human subject; tissue /cell culture; tissue resource /registry; transfection

The primary function of the Peripheral Stem Cell Core Laboratory will be to act as the conduit for the processing of reconstituting hematopoietic stem cells, isolated, purified and/or expanded from either human bone marrow, peripheral or placental blood. This central core laboratory will ensure the quality control of all stem cell materials distributed to other laboratories for research purposes, adherence to all current and future regulatory requirements and cost control for all projects, by bulk central purchasing agreements and by the efficient and integrated utilization of supplies. The Peripheral Stem Cell Core Laboratory (SCL) will have two interactive compounds, i) the Apheresis Unit to collect peripheral blood stem cells (PBSC) from normal donors and patients and ii) the Peripheral Stem Cell Research Laboratory. Apheresis services include the collection of peripheral blood stem cells from patients, the collection of allogeneic single donor platelets and peripheral blood stem cells for research purposes from normal volunteer donors. Services to be provided in the Peripheral Stem Cell Research Laboratory, include: i) Isolation, purification and ex-vivo expansion of stem cell populations from marrow, peripheral and placental blood, ii) Analysis of stem cell populations by flow cytometry and immunohistochemistry, iii) Cryopreservation and storage of stem cell populations for research use, iv) Disbursement of purified, expanded and/or cryopreserved stem cell populations for research purposes and v) Development and implementation of clinical protocols for gene transfer into hematopoietic stem cell populations.

外周干细胞核心实验室的主要功能是 作为处理重建造血细胞的管道 从人骨中分离、纯化和/或扩增的干细胞 骨髓、外周血或胎盘血。 这个中央核心实验室将 确保分发给的所有干细胞材料的质量控制 其他实验室用于研究目的，遵守所有当前和 所有项目的未来监管要求和成本控制（按批量） 中央采购协议以及高效、综合的 物资的利用。外周干细胞核心实验室（SCL） 将有两种相互作用的化合物，i）要收集的血浆分离装置 来自正常捐献者和患者的外周血干细胞（PBSC） ii) 外周干细胞研究实验室。 血浆分离术服务 包括从患者身上采集外周血干细胞、 同种异体单一供体血小板和外周血干细胞的采集 来自正常志愿者捐赠者的用于研究目的的细胞。 服务对象 由外周干细胞研究实验室提供，包括： i) 干细胞群的分离、纯化和离体扩增 来自骨髓、外周血和胎盘血，ii) 干细胞分析 通过流式细胞术和免疫组织化学分析群体，iii) 冷冻保存和储存干细胞群以供研究使用， iv) 纯化、扩增和/或冷冻保存干细胞的分配 用于研究目的的人群和 v) 开发和实施 基因转移至造血干细胞的临床方案 人口。