CORE--PERIPHERAL STEM CELL LABORATORY

核心--外周干细胞实验室

• 批准号: 3733465
• 负责人: Leonard Stanley Sender
• 金额: --
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3733465
• 关键词: biomedical facility; blood treatment; flow cytometry; hematopoietic stem cells; human subject; tissue /cell culture; tissue resource /registry; transfection; biomedical facility; blood treatment; flow cytometry; hematopoietic stem cells; human subject; tissue /cell culture; tissue resource /registry; transfection

The primary function of the Peripheral Stem Cell Core Laboratory will be to act as the conduit for the processing of reconstituting hematopoietic stem cells, isolated, purified and/or expanded from either human bone marrow, peripheral or placental blood. This central core laboratory will ensure the quality control of all stem cell materials distributed to other laboratories for research purposes, adherence to all current and future regulatory requirements and cost control for all projects, by bulk central purchasing agreements and by the efficient and integrated utilization of supplies. The Peripheral Stem Cell Core Laboratory (SCL) will have two interactive compounds, i) the Apheresis Unit to collect peripheral blood stem cells (PBSC) from normal donors and patients and ii) the Peripheral Stem Cell Research Laboratory. Apheresis services include the collection of peripheral blood stem cells from patients, the collection of allogeneic single donor platelets and peripheral blood stem cells for research purposes from normal volunteer donors. Services to be provided in the Peripheral Stem Cell Research Laboratory, include: i) Isolation, purification and ex-vivo expansion of stem cell populations from marrow, peripheral and placental blood, ii) Analysis of stem cell populations by flow cytometry and immunohistochemistry, iii) Cryopreservation and storage of stem cell populations for research use, iv) Disbursement of purified, expanded and/or cryopreserved stem cell populations for research purposes and v) Development and implementation of clinical protocols for gene transfer into hematopoietic stem cell populations.

外围干细胞核心实验室的主要功能将是 充当加工造血的渠道 干细胞，孤立，纯化和/或从任何人骨头扩张 骨髓，外周或胎盘血。 这个中央核心实验室将 确保所有分布到的干细胞材料的质量控制 用于研究目的的其他实验室，遵守所有当前和 所有项目的未来监管要求和成本控制，批量 中央采购协议以及高效和集成的 用品的利用。外围干细胞核心实验室（SCL） 将有两个交互式化合物，i）用于收集 正常供体和患者的外周血干细胞（PBSC）， ii）周围干细胞研究实验室。 语音服务 包括收集患者的外周血干细胞， 集合单一供体血小板和外周血茎的收集 用于研究目的的细胞来自正常的志愿捐助者。 服务 在外围干细胞研究实验室中提供： i）干细胞种群的隔离，纯化和离体扩展 来自骨髓，外周和胎盘血，ii）干细胞的分析 流式细胞仪和免疫组织化学的种群，iii） 干细胞群的冷冻保存和存储用于研究用途， iv）纯化，膨胀和/或冷冻保存的干细胞支出 研究目的的人口和v）开发和实施 基因转移到造血干细胞的临床方案 人群。