Biogeochemical cycling of N-osmolytes in the surface ocean

表层海洋中氮渗透剂的生物地球化学循环

• 批准号: NE/M002233/1
• 负责人: Yin Chen
• 金额: $ 45.31万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FM002233%2F1
• 关键词: Biogeochemical; cycling; osmolytes; surface; ocean

Nitrogen-containing compounds, including glycine betaine (GBT), choline and trimethylamine N-oxide (TMAO) are ubiquitous in marine organisms. They are used by marine organisms as compatible solutes in response to changes in environmental conditions, such as increasing salinity, because they do not interfere with cell metabolism. They also have beneficial effects in protecting proteins against denaturation due to chemical or physical damage.In the marine environment, these compounds are frequently released from these organisms directly into seawater due to changing environmental conditions, such as by viral lysis or grazing. The released nitrogenous osmolytes serve as important nutrients for marine microorganisms, which can use them as carbon, nitrogen and energy sources. It is well known that the degradation of these nitrogenous osmolytes contribute to the release of climate-active gases, including volatile methylated amines. Methylated amines are important sources of aerosols in the marine atmosphere, which help to reflect sunlight and cause a cooling effect on the climate. There is an urgent need to understand the microbial metabolism of these compounds and their seasonal cycles in the marine water column, in order to better understand their role in marine biogeochemical cycles and their role in future climate change.Built on the recent progress of the discovery of a new pathway of TMAO degradation in marine organisms and the development of a powerful liquid chromatography with mass spectrometry (LC-ESI-MS) method for simultaneous quantification of these nitrogenous osmolytes from the applicants' laboratories, this timely proposal aims to determine the seasonal cycle of nitrogenous osmolytes in surface seawater and to address how these environmentally-relevant compounds are degraded and what are the major microorganisms that are involved in the process. The data generated will fill in a major gap in our knowledge of the marine carbon and nitrogen cycles and the contribution of these compounds in future climate change through the release of climate-active molecules. Using the newly developed analytic techniques, we aim to determine the seasonal cycle of standing concentrations of nitrogenous osmolytes in the surface seawater and microbial oxidation activities. These data will be incorporated into a biogeochemical model for future prediction of biogeochemical cycles of N-osmolytes under climate change. Using cultivated model organisms, we aim to define the key genes, enzymes and the metabolic pathways in GBT and TMAO degradation by marine planktonic microbes.Using molecular and single cell manipulation techniques, we aim to further determine the key microbial players involved in the metabolism of nitrogenous osmolytes in surface seawater from the English Channel.This work will generate novel knowledge about our understanding of the microbial transformation of these nitrogen containing compounds, and will fill a serious gap in knowledge of marine carbon and nitrogen cycles. The project is expected to further strengthen the UK as a leading country not only in research of marine biogeochemical cycles and marine microbiology, but also in the development of cutting edge technology in environmental science.

含氮的化合物，包括甘氨酸甜菜碱（GBT），胆碱和三甲胺N-氧化物（TMAO）在海洋生物中无处不在。它们被海洋生物用作兼容溶质，以响应环境条件的变化（例如盐度增加），因为它们不会干扰细胞代谢。它们在保护蛋白质免受化学或物理损害引起的非变性方面也具有有益的作用。在海洋环境中，由于环境条件的变化，例如通过病毒裂解或放牧，这些化合物经常直接从这些生物中释放到海水中。释放的氮渗透剂是海洋微生物的重要营养素，可以将其用作碳，氮和能源。众所周知，这些氮渗透剂的降解有助于释放气候活性气体，包括挥发性甲基化胺。甲基化胺是海洋大气中气溶胶的重要来源，有助于反映阳光并对气候产生冷却作用。 There is an urgent need to understand the microbial metabolism of these compounds and their seasonal cycles in the marine water column, in order to better understand their role in marine biogeochemical cycles and their role in future climate change.Built on the recent progress of the discovery of a new pathway of TMAO degradation in marine organisms and the development of a powerful liquid chromatography with mass spectrometry (LC-ESI-MS) method为了同时量化申请人实验室的这些氮渗透液，该及时的建议旨在确定地表海水中氮渗透液的季节性周期，并解决这些与环境相关的化合物如何降解以及在此过程中涉及的主要微生物。生成的数据将填补我们对海洋碳和氮循环的了解，以及通过释放气候活性分子在未来气候变化中的贡献。我们使用新开发的分析技术，旨在确定地表海水和微生物氧化活性中氮渗透剂的静置浓度的季节性周期。这些数据将纳入一个生物地球化学模型中，以未来预测气候变化下N-渗透剂的生物地球化学周期。我们旨在使用培养的模型生物体来定义GBT中的关键基因，酶和代谢途径，并通过海洋浮游物微生物来定义GBT和TMAO降解。使用分子和单个细胞操纵技术，我们旨在进一步确定硝酸源源物的新氮的代谢范围的关键微生物参与者。这些含有化合物的氮的微生物转化，将填补对海洋碳和氮循环的认识的严重空白。预计该项目将进一步加强英国作为海洋生物地球化学周期和海洋微生物学研究的领先国家，而且还在环境科学领域的尖端技术发展中。

项目成果

Aminolipids elicit functional trade-offs between competitiveness and bacteriophage attachment in Ruegeria pomeroyi.

• DOI: 10.1038/s41396-022-01346-0
• 发表时间: 2023-03
• 期刊: ISME JOURNAL
• 影响因子: 11
• 作者: Stirrup, Rachel;Mausz, Michaela A.;Silvano, Eleonora;Murphy, Andrew;Guillonneau, Richard;Quareshy, Mussa;Rihtman, Branko;Ferretjans, Maria Aguilo;He, Ruo;Todd, Jonathan D.;Chen, Feng;Scanlan, David J.;Chen, Yin
• 通讯作者: Chen, Yin

Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation.

• DOI: 10.3389/fmicb.2020.552135
• 发表时间: 2020
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Silvano E;Yang M;Wolterink M;Giebel HA;Simon M;Scanlan DJ;Zhao Y;Chen Y
• 通讯作者: Chen Y

Lipid remodelling is a widespread strategy in marine heterotrophic bacteria upon phosphorus deficiency.

• DOI: 10.1038/ismej.2015.172
• 发表时间: 2016-04
• 期刊: The ISME journal
• 作者: Sebastián M;Smith AF;González JM;Fredricks HF;Van Mooy B;Koblížek M;Brandsma J;Koster G;Mestre M;Mostajir B;Pitta P;Postle AD;Sánchez P;Gasol JM;Scanlan DJ;Chen Y
• 通讯作者: Chen Y

Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade.

• DOI: 10.1111/1462-2920.12943
• 发表时间: 2015-12
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Lidbury I;Kimberley G;Scanlan DJ;Murrell JC;Chen Y
• 通讯作者: Chen Y

DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms.

• DOI: 10.1007/978-1-4939-6691-2_5
• 发表时间: 2017
• 期刊: Methods in molecular biology
• 作者: E. Jameson;M. Taubert;Sara Coyotzi;Yin Chen;Ö. Eyice;H. Schäfer;J. Murrell;J. Neufeld;M. Dumont