AGING ON GENE EXPRESSION OF GROWTH FACTORS IN NORMAL TRABECULAR OSTEOBLASTS

正常小梁成骨细胞生长因子基因表达的老化

• 批准号: 6264949
• 负责人: B. LAWRENCE RIGGS
• 金额: $ 2.01万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6264949
• 关键词: aging; clinical research; female; fibroblast growth factor; gene expression; growth factor; human subject; insulinlike growth factor; osteoblasts; osteogenesis; transforming growth factors

The purpose of this study is to define the mechanism of decrease in osteoblastic activity at the cellular level, which is principally responsible for the slow, age-related phase of bone loss. Our central hypothesis is that age-related impairment in the production of key growth factors in the bone microenvironment is responsible for most or all of the impaired osteoblast function. We have developed state-of-the-art methods for isolating human trabecular osteoblasts in cancellous bone and for measuring the small concentration of mRNA for the relevant growth factors. In the proposed studies, we will compare steady-state levels of mRNA for the relevant growth factors. In the proposed studies, we will compare steady-state levels of mRNA for IGF-I, IGF-II, TGFB, and bFGF in osteoblasts from a group of young normal adult women with those from a group of elderly normal women to test our hypothesis.

本研究的目的是确定细胞水平上成骨细胞活性降低的机制，这主要是导致缓慢的、与年龄相关的骨质流失阶段的原因。 我们的中心假设是，与年龄相关的骨微环境中关键生长因子的产生受损是大部分或全部成骨细胞功能受损的原因。 我们开发了最先进的方法来分离松质骨中的人小梁成骨细胞并测量相关生长因子的小浓度 mRNA。在拟议的研究中，我们将比较相关生长因子的 mRNA 稳态水平。 在拟议的研究中，我们将比较一组年轻正常成年女性和一组老年正常女性成骨细胞中 IGF-I、IGF-II、TGFB 和 bFGF 的稳态 mRNA 水平，以检验我们的假设。