MECHANISMS OF IMPLANTATION OF BLASTOCYST

囊胚植入机制

• 批准号: 6116646
• 负责人: ALLEN C ENDERS
• 金额: $ 5.91万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116646
• 关键词: animal tissue; growth /development; reproductive system

Significance Implantation of the blastocyst in the maternal endometrium follows a specific sequence in different species of mammals. Nonhuman primates show similarities between species and with the human with regard to syncytial trophoblast formation, formation of the trophoblastic plate, and lacunar and villus formation. We have determined that the cytotrophoblast involved in formation of the trophoblastic shell demonstrates surface features which appear to enable this trophoblast to invade maternal arteries as well as endometrial stroma. Objectives The objective of this project is to understand more completely the processes involved in implantation of the blastocyst in the endometrium in non-human primates. Studies focus on cellular interactions of the trophoblast and endometrium, and on differentiation of the early post implantation embryo and trophoblast. Results Macaque implantation sites were examined by electron microscopy and immuno-cytochemistry, focussing on modification of the wall of maternal arteries by endovascular cytotrophoblast. Immunostaining of cell adhesion molecules combined with localization of constituents that indicate cell type showed a sequence of changes in the vessel wall as cytotrophoblast migrated and invaded. Electron microscopic examination of the vessels showed that intramural cytotrophoblast secretes a matrix which excludes maternal elements, resulting in an intramural pad of cytotrophoblast. The studies also described the way in which the arteries, especially the muscle layers of the vessels, become remodeled. Future Directions Although cytotrophoblast modifies maternal arteries, the first invasion of the uterine epithelium is by syncytial trophoblast. It is not yet known what cell surface factors facilitate invasion of this epithelium and of maternal vessels. We will attempt to obtain some pre-lacunar implantation stages in order to assess the invasive characteristics of trophoblast types in the first days of implantation. KEYWORDS placenta, trophoblast, endometrium, immunocytochemistry FUNDING NIH Grant HD10342

胚泡在母体中的显着性植入 子宫内膜遵循不同物种的特定序列 哺乳动物。 非人类灵长类动物在物种和与 人类关于合成滋养细胞的形成，形成 滋养细胞板，lacunar和绒毛的形成。 我们有 确定涉及形成的细胞增生细胞 滋养细胞外壳展示了表面特征 启用此滋养细胞入侵产妇动脉以及 子宫内膜基质。 目标的目的是 更完全了解植入的过程 非人类灵长类动物子宫内膜中的胚泡。 研究 专注于滋养细胞和子宫内膜的细胞相互作用，以及 关于早期植入后胚胎的区分和 滋养细胞。 结果通过 电子显微镜和免疫循环化学，重点放在 通过血管内修饰孕产妇动脉壁 细胞营养细胞。 细胞粘附分子的免疫染色结合 指示细胞类型的成分的定位显示 随着细胞增生层细胞的迁移，血管壁变化的顺序 入侵。 电子的电子显微镜检查表明 壁内细胞增生细胞分泌一个不包括母体的基质 元素，导致壁内垫的细胞增生层。 这 研究还描述了动脉，尤其是 血管的肌肉层被重塑。 未来的方向 尽管细胞营养细胞修饰了母体动脉，但第一个 子宫上皮的侵袭是通过合成滋养细胞的。 这是 尚不知道哪些细胞表面因素有助于入侵 上皮和母体血管。 我们将尝试获得一些 诊断前植入阶段以评估侵入性 滋养细胞类型的特征在第一天 植入。 关键词胎盘，滋养细胞，子宫内膜， 免疫细胞化学资金NIH Grant HD10342