MECHANISMS OF IMPLANTATION OF THE BLASTOCYST

囊胚植入机制

基本信息

批准号：
2196717
负责人：
ALLEN C ENDERS
金额：
$ 23.36万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
起止时间：
1977-04-01 至 1999-03-31
项目状态：
已结题

项目摘要

Cytological, immunohistological and immunocytological methods will be used to investigate the role of different trophoblast populations during implantation in the macaque, and to compare these observations with those in two species, the rat and horse, exhibiting different patterns of epithelial and stromal invasion. The role of fetal-maternal cell fusion in expansion of trophoblast along the plane of the epithelium will be studied in the macaque using cytological and cell marker techniques. The extent of linkage between cytotrophoblast cells and cytotrophoblast cells and syncytial trophoblast will be studied using antibodies to junctional complex constituent proteins in this species. DNA synthesis in trophoblast populations will be examined using 3H thymidine incorporation in vitro in macaque implantation sites. The role of selected cell adhesion molecules in interaction of trophoblast and uterine tissues will be followed using antibody methods during implantation and early placentation in the macaque and rat. As a means of characterizing different trophoblast populations and providing functional implications the distribution of lysosomal and nonlysosomal lytic enzymes will be mapped in macaque trophoblastic plate to anchoring villous stages. Surface modifications and differentiation of equine girdle cells will be examined as they relate to migration of these cells into the endometrium. Adhesion molecules also will be studied in relationship to segregating cell populations in the uterus and trophoblast of the rat during implantation and early placentation. Adhesion molecules between trophoblast cells and endothelial cells will be localized to establish their role in formation of vascular patterns in the macaque and rat, and the manner in which equine trophoblast cells invade lymphatic vessels but not capillaries will be examined. Finally, embryos obtained during the macaque studies will be used to examine the manner of formation of the secondary yolk sac and the contribution of the prochordal plate and notochordal process to the establishment of definitive intraembryonic endoderm. It is believed that these studies can make a substantial contribution to our understanding of the way in which trophoblast cells can interact with the endometrium during the crucial implantation stages preceding placentation.

细胞学，免疫组织学和免疫细胞学方法将是用于研究不同滋养细胞种群的作用将猕猴植入，并将这些观察结果与这些观察结果进行比较在两个物种中，大鼠和马展示了不同的模式上皮和基质入侵。胎儿母细胞融合的作用在扩大滋养细胞的沿着上皮平面的扩展为使用细胞和细胞标记技术在猕猴中进行了研究。这细胞增生细胞和细胞增生细胞细胞之间的连接程度和合成滋养细胞将使用抗体的抗体进行研究该物种中的复杂成分蛋白。 DNA合成将使用3H胸苷掺入来检查滋养细胞种群猕猴植入部位的体外。选定细胞的作用滋养细胞和子宫组织相互作用中的粘附分子将在植入和早期使用抗体方法遵循猕猴和大鼠的胎盘。作为表征的一种手段不同的滋养细胞种群并提供功能含义溶酶体和非溶酶体裂解酶的分布将是在猕猴的滋养板上映射到固定绒毛阶段。马腰带细胞的表面修饰和分化将是在与这些细胞迁移到子宫内的迁移时进行了检查。还将研究与分离的关系的粘附分子大鼠子宫和滋养细胞中的细胞群体在植入和早期胎盘。之间的粘附分子滋养细胞和内皮细胞将被定位以建立它们在猕猴和大鼠中血管模式形成中的作用，以及马匹滋养细胞侵入淋巴管但不会检查毛细血管。最后，在猕猴研究将用于检查形成方式次级蛋黄囊和序板的贡献和确定确定性内部的脊索过程内胚层。据信，这些研究可以实现对我们对滋养细胞细胞方式的理解的贡献可以在关键植入阶段与子宫内膜相互作用胎盘之前。