CORE--DNA SEQUENCING FACILITY

核心——DNA测序设备

• 批准号: 6396720
• 负责人: ROBERT HASELKORN
• 金额: --
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6396720
• 关键词: DNA; biomedical facility; nucleic acid sequence

The University of Chicago Cancer Research Center DNA Sequencing Facility provides automated DNA sequencing services. The goals of the Facility are to provide accurate sequences at the lowest cost and the fastest turn- around time possible. The Facility includes an ABl 377 automated sequenator, 92-well and 48-well thermal cyclers, a Macintosh computer, a high resolution printer, and several software packages for sequence editing and alignment, including the latest version of Sequencer form Gene Codes. Users obtain order forms from the Facility World Wide Web page, fill them out (including the names and descriptions of each DNA template to be sequenced), place them in a personal file at the Facility, and then deliver a hard copy of the order together with the DNA samples. Facility technicians run the cycle sequencing reactions, usually dye- terminator reactions with primers supplied by the users, and then the sequencing gels, each with 36 lanes. The resulting chromatograms are placed in each user file, from which they can be down-loaded for editing and alignment. Turn-around time is 3-4 days. Routine results give good sequence for 600 bases; some templates can be read beyond 800. Facility staff provide users with advice on cloning vectors, template preparation, and planning of sequencing projects.

芝加哥大学癌症研究中心DNA测序设施 提供自动DNA测序服务。该设施的目标是 以最低的成本和最快的转盘提供准确的序列 可能的时间可能。该设施包括ABL 377自动化 序列，92孔和48孔热循环器，一台Macintosh计算机，一个 高分辨率打印机和序列的几个软件包 编辑和对齐，包括最新版本的音序器表单 基因代码。用户从全球网络中获取订单表格 页面，填写（包括每个DNA的名称和描述 要测序的模板），将它们放在设施的个人文件中， 然后与DNA样品一起提供订单的硬拷贝。 设施技术人员运行周期测序反应，通常是染料 用户提供的引物的终结者反应，然后 测序凝胶，每个凝胶都有36个车道。由此产生的色谱图是 放置在每个用户文件中，可以从中下载以进行编辑 和对齐。转机时间为3-4天。常规结果给予好 600个基部的序列；一些模板可以读取800以上。设施 员工为用户提供有关克隆向量，模板准备的建议， 和测序项目的计划。