INFRARED MICROSPECTROSCOPE WITH 100 NM RESOLUTION

分辨率为 100 nm 的红外显微镜

• 批准号: 6056757
• 负责人: SHYAMSUNDER ERRAMILLI
• 金额: $ 12.23万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6056757
• 关键词: atherosclerotic plaque; atomic force microscopy; bacteriorhodopsins; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; confocal scanning microscopy; cytology; imaging /visualization /scanning; infrared microscopy; infrared spectrometry; lasers; water solution

DESCRIPTION (Adapted from the applicant's abstract): The applicant proposes the construction of a table top infrared microspectrometer that is capable of obtaining infrared spectra from a lOO nm X lOO nm region of a biological specimen in an aqueous medium. This represents an improvement of a factor of 4,000 over what commercial infrared microscopes are capable of accomplishing with conventional light sources. It also represents an improvement of roughly 30 below the diffraction limit. At this resolution, it should be possible to characterize sub-cellular organelles without having to use any stains or fluorescent labels, relying on the intrinsic vibrational absorption spectrum of the constituent biomolecules. The new infrared microscope depends on the availability of new tunable infrared laser sources, allowing for new instruments that previously required accelerator-based lasers. In collaboration with investigators at Princeton University, Stanford University and Boston University, the investigators have recently developed the world's first scanning near field infrared microscope based on free electron laser (FEL) to image biological samples. This new microscope has demonstrated that it is possible to break the diffraction limit in the infrared part of the spectrum, and has been used to acquire infrared images of both semiconductor and biological tissue sections. Most promising is the ability to obtain, for the first time, infrared images under water. The investigators propose that a table-top version of the new microscope can be constructed that can result in vibrational spectra from a single location in a sample on length scales that are unprecedented. The proposal requires a confluence of novel developments in many fields, relying on breakthroughs in lasers, scanning probe microscopes, new materials, and the adaptation of all of this to biological samples. At the same time, the payoff of the new technique is also extremely promising, because of its reliance on the intrinsic spectral characteristics of biomolecules, and not on externally applied stains or perturbing labels.

描述（根据申请人的摘要改编）：申请人提出 构建能够 从生物学的LOO NM X LOO NM区域获得红外光谱 在水性介质中的标本。 这代表了一个因素的改善 在4,000个商业红外显微镜的能力上的4,000个 使用传统的光源来完成。 它也代表 在衍射极限以下的改善大约30。 在此决议中， 应该可以表征下细胞细胞器而不 使用任何污渍或荧光标签，依靠固有 组成生物分子的振动吸收光谱。 新的 红外显微镜取决于新可调红外的可用性 激光来源，允许以前需要的新仪器 基于加速器的激光器。 与普林斯顿的调查员合作 大学，斯坦福大学和波士顿大学，调查人员 最近开发了世界上首次扫描近红外线 基于游离电子激光（FEL）的显微镜图像生物样品。 这个新的显微镜表明，有可能打破 频谱红外部分的衍射极限，已用于 获取半导体和生物组织的红外图像 部分。 最有前途的是第一次获得的能力 红外图像在水下。 调查人员建议一个桌面 可以构造新显微镜的版本，可以导致 从样本中的单个位置的振动光谱，长度尺度上 是前所未有的。 该提案需要新的发展融合 在许多领域，依靠激光的突破，扫描探针 显微镜，新材料以及所有这些对生物学的适应 样品。 同时，新技术的收益也是 由于它依赖固有光谱，因此极为有希望 生物分子的特征，而不是外部施用的污渍或 扰动标签。