COMPUTATIONAL METHODS FOR ANALYSIS OF NEUROIMAGING DATASETS

神经影像数据集分析的计算方法

• 批准号: 6121986
• 负责人: NIGEL H GODDARD
• 金额: $ 0.9万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121986
• 关键词: bioimaging /biomedical imaging; biomedical resource; computers; microscopy; technology /technique

The overall mission of the Developmental Resource for Biophysical Imaging and Opto-Electronics (DRBIO) consists of the creation, development and application of new instrumentation and analysis technologies for the visualization and measurement of dynamic cellular and molecular processes. Our current primary research areas are the development of Multi-Photon Excitation Laser Scanning Microscopy (MPELSM), a technique first demonstrated by our group in 1990, and applications of Fluorescence Correlation Spectroscopy (FCS), a technique also developed in this group 25 years ago which has recently found many new uses with the advent of inexpensive and powerful computers. The advantages of fluorescence imaging with multiphoton excitation include a substantial reduction of photobleaching and photodamage outside of the focal planes (due to the intensity squared dependence of the focused beam), deeper penetration of the excitation beam into thick, scattering samples, and the ability to excite UV dyes without the need for UV optics. We are an NCRR Resource and carry out collaborations with researchers in animal and plant biology, chemistry, physics and medicine here at Cornell and from other national and international universities. Our invention of the MPELSM has recently been licensed by BioRad Life Science's microscopy division and is now a commercial product. With assistance from Parallel Processing Resource for Biomedical Scientists at the Cornell Theory Center, collaborators and staff at DRBIO have developed routine methods using IBM Data Explorer software for the reconstruction and animation of 3D images from multiple image sections through a specimen.

生物物理发展资源的总体使命 成像和光电（DRBIO）由创作组成， 新仪器和分析的开发和应用 可视化和测量动态细胞的技术 和分子过程。 我们目前的主要研究领域是 多光子激发激光扫描显微镜的开发 （mpelsm），我们的小组于1990年首次证明的技术， 荧光相关光谱（FCS）的应用，A 技术还在25年前的该组中开发了 在廉价且强大的出现时发现了许多新用途 计算机。 荧光成像的优势使用多光子 激发包括大幅减少光漂白和 焦平面外的光损伤（由于强度平方 聚焦光束的依赖性），更深的激发渗透 横梁成厚的散射样品，并激发紫外染料的能力 无需紫外光学。 我们是NCRR资源，并执行 与动物和植物生物学研究人员合作， 康奈尔的化学，物理和医学以及其他 国家和国际大学。 我们对MPELSM的发明 最近已获得Biorad Life Science的显微镜许可 部门，现在是商业产品。 在来自的协助下 康奈尔的生物医学科学家的并行处理资源 理论中心，DRBIO的合作者和员工已经开发了常规 使用IBM数据Explorer软件进行重建和 来自多个图像部分的3D图像的动画通过 标本。