COMPUTATIONAL METHODS FOR ANALYSIS OF NEUROIMAGING DATASETS

神经影像数据集分析的计算方法

• 批准号: 6121986
• 负责人: NIGEL H GODDARD
• 金额: $ 0.9万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121986
• 关键词: bioimaging /biomedical imaging; biomedical resource; computers; microscopy; technology /technique

The overall mission of the Developmental Resource for Biophysical Imaging and Opto-Electronics (DRBIO) consists of the creation, development and application of new instrumentation and analysis technologies for the visualization and measurement of dynamic cellular and molecular processes. Our current primary research areas are the development of Multi-Photon Excitation Laser Scanning Microscopy (MPELSM), a technique first demonstrated by our group in 1990, and applications of Fluorescence Correlation Spectroscopy (FCS), a technique also developed in this group 25 years ago which has recently found many new uses with the advent of inexpensive and powerful computers. The advantages of fluorescence imaging with multiphoton excitation include a substantial reduction of photobleaching and photodamage outside of the focal planes (due to the intensity squared dependence of the focused beam), deeper penetration of the excitation beam into thick, scattering samples, and the ability to excite UV dyes without the need for UV optics. We are an NCRR Resource and carry out collaborations with researchers in animal and plant biology, chemistry, physics and medicine here at Cornell and from other national and international universities. Our invention of the MPELSM has recently been licensed by BioRad Life Science's microscopy division and is now a commercial product. With assistance from Parallel Processing Resource for Biomedical Scientists at the Cornell Theory Center, collaborators and staff at DRBIO have developed routine methods using IBM Data Explorer software for the reconstruction and animation of 3D images from multiple image sections through a specimen.

生物物理学发展资源的总体使命 成像和光电 (DRBIO) 包括创造、 新型仪器和分析的开发和应用 动态细胞可视化和测量技术 和分子过程。 我们目前的主要研究领域是 多光子激发激光扫描显微镜的发展 （MPELSM），这是我们小组于 1990 年首次展示的技术，以及 荧光相关光谱（FCS）的应用 25 年前，该小组也开发了该技术，该技术最近已 随着廉价且功能强大的产品的出现，发现了许多新用途 电脑。 多光子荧光成像的优点 激发包括光漂白的显着减少和 焦平面外的光损伤（由于强度平方 聚焦光束的依赖性），激发的更深穿透 光束射入厚的散射样品，并能够激发紫外染料 无需紫外光学器件。 我们是 NCRR 资源并执行 与动植物生物学研究人员合作， 康奈尔大学和其他大学的化学、物理和医学 国内和国际大学。 我们发明的 MPELSM 最近获得 BioRad Life Science 显微镜授权 部门，现已成为商业产品。 在来自的帮助下 康奈尔大学生物医学科学家的并行处理资源 理论中心、DRBIO 的合作者和工作人员已经制定了常规 使用 IBM Data Explorer 软件进行重建的方法 来自多个图像部分的 3D 图像的动画 标本。