MEMBRANE DYNAMICS

膜动力学

• 批准号: 6206157
• 负责人: DANIEL LARSON
• 金额: $ 1.75万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206157
• 关键词: bioengineering /biomedical engineering; biological products; biomedical resource; growth /development; lasers; plants; technology /technique

By labeling the soluble matrix (the stroma) of tobacco cell plastids with green fluorescent protein, it was possible in previous work to visualize thin tubular projections which emanate from the plastid surface and sometimes interconnect with other plastids, as well as to demonstrate that interconnected plastids can exchange protein through these plastid tubules (Khler et al. 1997). FCS provides a means to characterize the transport properties of such interplastid protein exchange. The effect of inhibitors like Cyanide, Colchicin and Cytochalesin D on the transport parameters is tested and quantified.

通过标记烟草细胞的可溶性基质（基质） 带有绿色荧光蛋白的质体，在以前是可能的 致力于可视化从 质体表面，有时与其他质体互连，如 以及证明相互连接的质体可以交换 蛋白质通过这些质体小管（K hler et al. 1997）。 燃料电池系统 提供了一种表征此类传输特性的方法 质体间蛋白质交换。 氰化物等抑制剂的作用， 秋水仙素和细胞松弛素 D 对运输参数的影响进行了测试 量化。