FLOW CYTOMETER TO UPGRADE AND EXPAND THE CORE FACILITY

流式细胞仪升级和扩建核心设施

• 批准号: 2791055
• 负责人: JAMES E TALMADGE
• 金额: $ 17.34万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791055
• 关键词: biomedical equipment; biomedical equipment purchase; cell sorting; flow cytometry

This proposal is an application to obtain a new multi-purpose multi-user flow cytometer, Becton Dickinson FACSCalibur with FL4 and sorting capabilities to provide adequate backup for the FACStar/PLUS. In addition, the following accessories are requested: FACSLoader and Barcode Scanner to increase the throughput of samples brought to the Cell Analysis (Facility). Increased sorting requests are over-committing the use of our FACStar/PLUS making it difficult to acquire data for routine immunofluorescent assays. In addition, any down time on the single flow cytometer available for research acquisition prevents results and unacceptable delays in acquisition. The primary users of the CAF have participated in this application and strongly support the request. An additional instrument for acquisition of multi-parametric data is needed to support ongoing studies and to facilitate research strategies for the successful competition for new and continuing NIH supported research at our institution. In addition, a benchtop flow cytometer such as the Becton Dickinson FACSCalibur would provide additional sorting time on the FACStar/Plus. Further, since both the FACStar/PLUS and the FACSCalibur utilize the same software for data collection and analysis, particularly the Becton Dickinson Attractors Program for multi-color analyses, retraining many of the users would be unnecessary. The researchers who will employ this instrument are supported by many NIH institutions and have an excellent record of prior and current funding. We also are acutely aware of the rapid growth of "high-tech" applications in modern basic and clinical research and expect to add additional NIH supported users to the user group. We encourage both NIH supported and scientists supported by other mechanisms to take advantage of the facility as time and availability permit in the interest of enhancing their potential for future NIH funding. The successful, but now overextended use of our current instrument has given us both the experience and desire to significantly upgrade our Core Facility. Administratively, the Flow Cytometry Advisory Committee, an experienced director, a well trained and experienced flow sorter operator, will allow rapid and efficient application of the new flow cytometer in ongoing and future projects.

该建议是为了获得新的多用途多用户流式细胞仪，具有FL4的Becton Dickinson Facscalibur和分类功能，以为Facstar/Plus提供足够的备份。此外，请求以下配件：FACSLOADER和条形码扫描仪以增加带到细胞分析（设施）的样品的吞吐量。增加的分类请求过于征收我们的FACSTAR/PLUS的使用，因此很难获取常规免疫荧光测定的数据。此外，可用于研究采集的单流动细胞仪上的任何停机时间都可以防止结果和无法接受的延迟。 CAF的主要用户参与了此应用程序，并强烈支持该请求。需要获得多参数数据的另一种工具来支持正在进行的研究，并促进研究策略，以成功地竞争我们机构的新的和持续的NIH支持研究。此外，台式流式细胞仪（例如Becton Dickinson facscalibur）将在Facstar/Plus上提供额外的分类时间。此外，由于FACSTAR/PLUS和FACSCALIBUR都使用相同的软件进行数据收集和分析，尤其是Becton Dickinson吸引者计划进行多色分析计划，因此不必要进行许多用户。将使用该工具的研究人员得到许多NIH机构的支持，并具有出色的先前和当前资金记录。我们还敏锐地意识到现代基础和临床研究中“高科技”应用程序的快速增长，并希望将其他支持用户添加到用户组中。我们鼓励支持NIH的支持和其他机制支持的科学家，以利用该设施，因为时间和可用性许可，以增强其未来NIH资金的潜力。成功的，但现在过度扩展我们当前的工具的使用使我们既可以显着升级我们的核心设施的经验和愿望。在行政上，流式细胞仪咨询委员会是一位经验丰富的经验丰富且经验丰富的流动分散器操作员，将允许在正在进行的和未来的项目中快速有效地应用新的流式细胞仪。