MOLECULAR ANALYSIS OF HEMATOPOIESIS

造血的分子分析

• 批准号: 6201939
• 负责人: LEROY E HOOD
• 金额: $ 19.7万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201939
• 关键词: Drosophilidae; animal genetic material tag; animal tissue; biomedical equipment development; biotechnology; cell population study; computer assisted sequence analysis; cytogenetics; gene expression; genetic library; genetic mapping; genetic markers; genetic strain; genetic techniques; genome; genotype; hematopoiesis; hematopoietic stem cells; laboratory mouse; linkage mapping; molecular cloning; nucleic acid hybridization; nucleic acid sequence; polymerase chain reaction; statistics /biometry

We will use genome-wide genetic mapping, large-scale DNA arrays and high-throughout DNA sequencing to identify murine candidate genes that may play a role in early hematopoiesis in determining the frequency of hematopoietic stem cells. We will also help clone and sequence Drosophila genes that may be homologues of those controlling early murine hematopoiesis. Candidate genes will be tested and biological assays developed by our collaborators.

我们将使用全基因组遗传图谱、大规模DNA 阵列和高通量 DNA 测序来识别小鼠 可能在早期造血过程中发挥作用的候选基因 确定造血干细胞的频率。 我们将 还有助于克隆和测序果蝇基因，这些基因可能是 控制早期小鼠造血的同源物。 将测试候选基因并开发生物测定法 我们的合作者。