ROLE OF CENTRAL CRF IN STRESS-INDUCED BEHAVIOR

中央 CRF 在压力诱发行为中的作用

• 批准号: 6186510
• 负责人: Ned H Kalin
• 金额: $ 24.02万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6186510
• 关键词: Animalia; amygdala; behavior; behavioral /social science research tag; corticotropin releasing factor; ethology; gene expression; hormone binding protein; hormone receptor; psychophysiology; stress

DESCRIPTION (adapted from applicant's abstract): Brain CRF systems are important in integrating endocrine, autonomic, and behavioral responses to stress. Preclinical and clinical research suggests an important involvement of CRF systems and the amygdala in mediating symptoms associated with anxiety disorders and depression. CRF containing cell bodies and fibers, as well as the CRF binding protein and CRF receptors are found throughout the amygdala. The investigators have found that the major site of outflow from the amygdala, the central nucleus (CeA), is a brain site where endogenous CRF systems mediate stress-induced behavioral responses. Using site specific administration of a CRF antagonist (CRFa), they demonstrated that CRF receptors in the region of the CeA mediate acute and conditioned freezing behavior. Complementing these studies, they demonstrated that acute stress exposure results in increased amygdala CRF mRNA concentrations. This is the first direct evidence demonstrating that stress activates amygdaloid CRF systems. The overall aim of this proposal is to continue studies examining the role of amygdaloid CRF systems in mediating behavioral responses to stress. The investigators will characterize the effects of stress on the activation of the genes for CRF, its receptor, and its binding protein. The studies will provide important hypotheses regarding the role of CRF systems in mediating pathological anxiety, and other forms of human psychopathology.

描述（改编自申请人的摘要）：Brain CRF 系统是 对于整合内分泌、自主神经和行为反应非常重要 压力。 临床前和临床研究表明重要的参与 CRF 系统和杏仁核在介导与 焦虑症和抑郁症。 CRF含有细胞体和纤维，如 以及 CRF 结合蛋白和 CRF 受体遍布于整个 杏仁核。 调查人员发现，主要流出地点是 杏仁核，即中央核 (CeA)，是大脑中内源性神经元的所在地 CRF 系统调节压力引起的行为反应。 使用网站 特定施用 CRF 拮抗剂 (CRFa)，他们证明 CeA 区域的 CRF 受体介导急性和条件性 冻结行为。 作为对这些研究的补充，他们证明了 急性应激暴露导致杏仁核 CRF mRNA 浓度增加。 这是第一个直接证据表明压力会激活 杏仁核 CRF 系统。 该提案的总体目标是继续 研究检查杏仁核 CRF 系统在调节行为中的作用 对压力的反应。 研究人员将描述其影响 应激对 CRF 基因、其受体及其结合的激活 蛋白质。 这些研究将提供有关角色的重要假设 CRF 系统在调节病理性焦虑和其他形式的人类焦虑中的作用 精神病理学。