CONTROLLING ALLERGENS AND COSTS IN THE ANIMAL FACILITY

控制动物设施中的过敏原和成本

• 批准号: 6188580
• 负责人: ABIGAIL L SMITH
• 金额: $ 32.06万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188580
• 关键词: Helicobacter; Mus musculus; Pasteurella; Pneumocystis carinii; SCID mouse; air filtration; air sampling /monitoring; airborne allergen; ammonia; animal care; animal colony; athymic mouse; communicable disease control; enzyme linked immunosorbent assay; epizootiology; germ free condition; immediate hypersensitivity; nonbiomedical equipment; occupational disease /disorder; occupational health /safety; polymerase chain reaction; postmortem; work site

DESCRIPTION (Adapted from the applicant's abstract): The overall goal of these studies is to establish cost-effective environmental conditions that support high standards of animal health and provide a comfortable, safe workplace. This application will test the animal health effects of changing animal husbandry procedures that are major sources of employee exposure to allergen. If these changes have no negative impact on animal health, instituting them should reduce allergen levels by more than 10-fold, and result in healthier workers and reduced animal care costs. By extrapolating the savings at TJL to all NIH-supported mouse care, the potential savings to NIH is $22 million a year. The first animal husbandry practice to be tested is housing animals in ventilated caging with cage pressure negative to the room and using enclosed negatively ventilated tables when changing cages. To measure allergen reduction, room and worker breathing zone allergen concentrations will be measured before and after the changes. Concern that cages with negative pressure might increase the transfer of pathogens among animals will be tested in an experimental animal room where infected mice will be isolated from other colonies. Transfer of Pneumocystis carinii from infected mice to pathogen-free immune-competent and immune-compromised mice will be monitored by several methods. The second animal husbandry change will be to reduce the frequency of cage changing from once every 7 days to once every 14 days. Allergen concentrations will be measured and compared for both changing schedules. The cage environment (ammonia, temperature, humidity) will be compared for each condition, and animal health will be assessed by reproductive performance, growth, immune system function, and by necropsy focused on the histology of the upper and lower respiratory systems of young mice. These studies will fill a number of gaps in scientific knowledge concerning the natural history of transmission of Pneumocystis among mice, and the harmful effects of ammonia in typical animal facilities where ammonia levels peak only for short times until bedding is changed or a litter is weaned. The use of mice naturally infected with P. carinii, Pasteurella pneumotropica and Helicobacter spp. will enable use of all three organisms in the negative pressure system.

描述（根据申请人的摘要改编）： 这些研究是建立具有成本效益的环境条件 支持高标准的动物健康，并提供舒适，安全的 工作场所。 该应用将测试动物健康变化的影响 畜牧程序是员工接触的主要来源 过敏原。 如果这些变化对动物健康没有负面影响， 使它们的过敏原水平应降低10倍以上，并且 导致更健康的工人和降低动物护理成本。 通过推断 TJL节省了所有NIH支持的鼠标护理，潜在的节省了 NIH每年为2200万美元。 第一个要测试的畜牧业习惯 在通风笼中外壳，笼子压力为笼子 更换笼子时，可以使用封闭式通风桌。 测量过敏原，房间和工人呼吸区过敏原 浓度将在更改之前和之后测量。 关心 负压负压的笼子可能会增加病原体在 动物将在被感染的小鼠的实验性动物室进行测试 将与其他殖民地隔离。 从肺炎雄叶carinii转移 感染的小鼠无病原体免疫能力和免疫受损小鼠 将通过几种方法监视。 第二个畜牧业改变 将是将笼子的频率从每7天降低一次 每14天一次。 过敏原浓度将被测量并比较 对于两个更改的时间表。 笼子环境（氨，温度， 将比较每种情况的湿度），动物健康将是 通过生殖性能，增长，免疫系统功能以及通过 尸检着重于上和下呼吸系统的组织学 年轻的老鼠。 这些研究将填补科学的许多空白 关于肺炎胸膜传播的自然历史的知识 在小鼠中，氨气在典型动物设施中的有害影响 氨水仅在短时间内达到峰值，直到替身或 垃圾是断奶的。 使用自然感染了carinii的小鼠， 巴斯德肺炎和螺旋杆菌属。 将实现所有人 负压系统中的三个生物。