MATERNAL CONTROL OF TISSUE FORMATION IN XENOPUS

爪蟾组织形成的母体控制

• 批准号: 6344181
• 负责人: JANET HEASMAN
• 金额: $ 3.62万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6344181
• 关键词: Xenopus oocyte; antisense nucleic acid; cell differentiation; developmental genetics; early embryonic stage; ectoderm; embryogenic cleavage; endoderm; genetic regulation; genomic imprinting; germ layers; histogenesis; mesoderm; neural plate /tube; nonmammalian vertebrate embryology; phenotype; transcription factor

All vertebrate embryos establish a basic body plan consisting of three germ layers, the ectoderm, mesoderm and endoderm, from which more complex tissues are derived. The molecular mechanism by which the basic plan is achieved is not yet clear. Recently, we have found that the maternal transcription factor VegT is an essential element in this process. We study its role by injecting antisense oligonucleotides complementary to VegT into oocytes and then fertilizing them and studying their development. In Aim 1, we aim to understand the VegT- depleted phenotype, by examining the process of gastrulation and neurulation in its absence, and by determining how the fate map is altered in these embryos. We will answer the important question of whether VegT is directly required for both mesoderm and endoderm formation. Aim 2 moves downstream of VegT, and asks what gene products lie downstream of VegT and can rescue the VegT-depleted phenotype. This aim uses both a candidate gene approach and subtractive screening. It also asks whether VegT expression causes the secretion of specific growth factors. In Aim 3, we will study the question of whether a previously described maternal signalling pathway, the dorsal pathway, which is responsible for converting mesodermal and ectodermal tissue to somites, notochord and neural tube, is still present in VegT-depleted embryos. It also turns to the question of what cell states remain when the VegT-initiated pathway and the dorsal pathway are deficient. This work will answer long-standing questions about how the body plan is set up in the early embryo, in particular providing the molecular framework for endoderm and mesoderm differentiation.

所有脊椎动物胚胎建立一个基本的身体计划，该计划由三个 细菌层，外胚层，中胚层和内胚层，从中更多 得出复杂的组织。 基本的分子机制 计划尚不清楚。 最近，我们发现 母体转录因子VEGT是这一点的重要因素 过程。 我们通过注射反义寡核苷酸来研究其作用 补充卵母细胞，然后将其施肥，然后 研究他们的发展。在AIM 1中，我们旨在了解VEGT- 通过检查胃术和 神经在缺席的情况下，并确定命运图是如何 这些胚胎改变了。 我们将回答一个重要的问题 中胚层和内胚层是否直接需要VEGT 形成。 AIM 2在VEGT的下游移动，并询问哪些基因产品 躺在VEGT的下游，可以挽救vegt缺乏的表型。这 AIM使用候选基因方法和减法筛查。 它 还询问VEGT表达是否引起特定的分泌 增长因素。 在AIM 3中，我们将研究一个问题 先前描述的母体信号通路，背途径， 负责转换中胚层和外胚层组织 对于Somites，Notochord和Neural Tube仍然存在于VEGT耗尽 胚胎。 它还转向一个问题，即当 VEGT引发的途径和背途径不足。 这 工作将回答有关如何设定身体计划的长期问题 在早期胚胎中，特别是提供分子框架 用于内胚层和中胚层分化。