IN VITRO SELECTION OF PROTEINS VIA MRNA-PROTEIN FUSIONS

通过 mRNA-蛋白质融合体外选择蛋白质

• 批准号: 6032513
• 负责人: RICHARD W ROBERTS
• 金额: $ 20.15万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6032513
• 关键词: G protein; aldehyde lyase; beta lactamase; biological signal transduction; catalyst; chimeric proteins; enzyme activity; genetic library; guanosinetriphosphatases; messenger RNA; peptides; protein binding; protein biosynthesis; protein sequence; protein structure function; ribosomes; tissue /cell culture

The experiments outlined in this proposal are designed to use in vitro selection experiments to explore protein structure, recognition, and catalysis. Because it is not currently possible to design a peptide or protein sequence that is imbued with desired functional properties, we have chosen to pursue an in vitro genetic strategy. We do this by using RNA-protein fusions, peptide or protein molecules covalently attached to the mRNA which encodes them. RNA-protein fusions allow for repeated rounds of selection and amplification of proteins because the coding and polypeptide sequences are united in a single molecule. This technique allows libraries containing more than 1013 different sequences to be generated in the total absence of a living cell. Thus, fusions provide a functional approach to protein design and afford selection where classical genetic screening cannot be applied. Our specific aims are: 1) To improve our understanding and implementation of protein selection using RNA-protein fusions. We have previously implemented RNA- protein fusions as a vehicle for in vitro peptide and protein selection. We propose to i) to examine the mechanism of fusion formation on the ribosome ii) to improve the synthesis and selection of fusion molecules, and iii), to explore protein design hypotheses in the construction of our combinatorial libraries. 2) To develop and explore peptides and proteins that modulate signal transduction pathways. We will use in vitro selection experiments to isolate peptides and proteins that mimic known regulators of G protein function. We will examine the affinity, specificity, and structure of our selected proteins with their target G protein, comparing the size and diversity of the molecules we isolate with the natural regulators. 3) To develop and explore methods to isolate novel catalyts in vitro. We will examine strategies to isolate novel peptide and protein catalysts. The diversity, mechanism, and structure of the sequences isolated will be explored with an eye toward fundamental questions of enzyme architecture. The information that will result from these experiments has tremendous potential to teach us about protein structure, recognition, and catalysis. In addition, our work should enable other laboratories to apply it as a general tool for protein discovery and dissection. The techniques used and specific molecules isolated should greatly facilitate the development of therapeutics for the treatment of human disease.

本提案中概述的实验旨在利用体外选择实验来探索蛋白质结构、识别和催化。 由于目前不可能设计出具有所需功能特性的肽或蛋白质序列，因此我们选择采用体外遗传策略。 我们通过使用 RNA-蛋白质融合体、肽或蛋白质分子共价连接到编码它们的 mRNA 来做到这一点。 RNA-蛋白质融合允许对蛋白质进行重复的选择和扩增，因为编码序列和多肽序列结合在单个分子中。该技术允许在完全没有活细胞的情况下生成包含超过 1013 个不同序列的文库。 因此，融合提供了一种功能性的蛋白质设计方法，并在经典遗传筛选无法应用的情况下提供了选择。 我们的具体目标是： 1) 提高我们对使用 RNA-蛋白质融合进行蛋白质选择的理解和实施。 我们之前已经将RNA-蛋白质融合作为体外肽和蛋白质选择的载体。 我们建议 i) 检查核糖体上融合形成的机制 ii) 改进融合分子的合成和选择，以及 iii) 在我们的组合文库构建中探索蛋白质设计假设。 2) 开发和探索调节信号转导途径的肽和蛋白质。 我们将使用体外选择实验来分离模拟已知 G 蛋白功能调节因子的肽和蛋白质。 我们将检查我们选择的蛋白质与其目标 G 蛋白的亲和力、特异性和结构，比较我们分离的分子与天然调节剂的大小和多样性。 3）开发和探索体外分离新型催化剂的方法。我们将研究分离新型肽和蛋白质催化剂的策略。 将着眼于酶结构的基本问题来探索分离序列的多样性、机制和结构。这些实验产生的信息具有巨大的潜力，可以帮助我们了解蛋白质结构、识别和催化。 此外，我们的工作应该使其他实验室能够将其应用为蛋白质发现和解剖的通用工具。 所使用的技术和分离的特定分子将极大地促进治疗人类疾病的疗法的开发。