UL33 GENE PRODUCT OF HSV1

HSV1 的 UL33 基因产物

• 批准号: 6228679
• 负责人: ASHLEY E REYNOLDS
• 金额: $ 0.86万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-26 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6228679
• 关键词: capsid; cell nucleus; chemical cleavage; herpes simplex virus 1; immunoprecipitation; intracellular transport; protein localization; protein protein interaction; protein transport; virus DNA; virus assembly; virus genetics; virus protein; yeast two hybrid system

DESCRIPTION Within the nuclei of herpes simplex virus (HSV-1) infected cells, replicating HSV-DNA forms head-to-tail concatemers which are cleaved to genome length molecules and subsequently translocated into performed capsids. The long term objective related to this proposal is the definition of the molecular mechanisms of HSV DNA cleavage and packaging. The HSV genes UL6, UL15, UL17, UL28, UL32, and UL33 are dispensable for capsid assembly, but have been shown to be necessary for viral DNA cleavage and packaging. UL33 is imported into the nucleus in infected cells. Importation is mediated by late gene product(s). The overall aim of this proposal is to characterize the role of UL33 protein in the HSV-1 packasome by 1) Identifying gene products required for viral UL33 nuclear important and 2) Identifying the critical protein domains necessary for protein-protein interactions between UL33 and other HSV proteins. We have eptiopically-tagged the UL33 gene product and developed a nuclear importation assay examining the effect of viruses lacking specific cleavage/packaging genes on nuclear importation of UL33 protein.

描述单纯疱疹病毒（HSV-1）感染细胞中的描述，复制HSV-DNA形成从头到尾的串联，它们被裂解至基因组长度分子并随后转移到执行的衣壳中。与该建议相关的长期目标是HSV DNA裂解和包装的分子机制的定义。 HSV基因UL6，UL15，UL17，UL28，UL32和UL33对于衣壳组件是可分配的，但已被证明是病毒DNA裂解和包装所必需的。 UL33在感染细胞中进口到核中。进口是由晚期基因产物介导的。该提案的总体目的是通过1）确定病毒ul33核的基因产物来表征UL33蛋白在HSV-1堆积体中的作用，并且2）确定UL33与其他HSV蛋白之间蛋白质蛋白质相互作用所必需的关键蛋白质结构域。我们已经用eptiopopage标记了UL33基因产物，并开发了一种核进口测定法，研究了缺乏特定裂解/包装基因对UL33蛋白核进口的病毒的作用。