UL33 GENE PRODUCT OF HSV1

HSV1 的 UL33 基因产物

• 批准号: 6315535
• 负责人: ASHLEY E REYNOLDS
• 金额: $ 4.96万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-26 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6315535
• 关键词: capsid; cell nucleus; chemical cleavage; herpes simplex virus 1; immunoprecipitation; intracellular transport; protein localization; protein protein interaction; protein transport; virus DNA; virus assembly; virus genetics; virus protein; yeast two hybrid system

DESCRIPTION Within the nuclei of herpes simplex virus (HSV-1) infected cells, replicating HSV-DNA forms head-to-tail concatemers which are cleaved to genome length molecules and subsequently translocated into performed capsids. The long term objective related to this proposal is the definition of the molecular mechanisms of HSV DNA cleavage and packaging. The HSV genes UL6, UL15, UL17, UL28, UL32, and UL33 are dispensable for capsid assembly, but have been shown to be necessary for viral DNA cleavage and packaging. UL33 is imported into the nucleus in infected cells. Importation is mediated by late gene product(s). The overall aim of this proposal is to characterize the role of UL33 protein in the HSV-1 packasome by 1) Identifying gene products required for viral UL33 nuclear important and 2) Identifying the critical protein domains necessary for protein-protein interactions between UL33 and other HSV proteins. We have eptiopically-tagged the UL33 gene product and developed a nuclear importation assay examining the effect of viruses lacking specific cleavage/packaging genes on nuclear importation of UL33 protein.

描述 在单纯疱疹病毒 (HSV-1) 感染细胞的细胞核内，复制的 HSV-DNA 形成头尾相连的多联体，这些多联体被切割成基因组长度的分子，随后易位到已加工的衣壳中。与该提案相关的长期目标是定义 HSV DNA 切割和包装的分子机制。 HSV 基因 UL6、UL15、UL17、UL28、UL32 和 UL33 对于衣壳组装来说是可有可无的，但已被证明对于病毒 DNA 切割和包装是必需的。 UL33 被输入到受感染细胞的细胞核中。输入是由晚期基因产物介导的。该提案的总体目标是通过以下方式表征 UL33 蛋白在 HSV-1 包装体中的作用：1) 鉴定病毒 UL33 核重要所需的基因产物；2) 鉴定 UL33 与其他蛋白之间的蛋白质-蛋白质相互作用所需的关键蛋白质结构域。 HSV 蛋白。我们对 UL33 基因产物进行了表位标记，并开发了一种核输入测定法，检查缺乏特定切割/包装基因的病毒对 UL33 蛋白核输入的影响。