MECHANISM OF CELL DIVISION PLANE PLACEMENT IN S POMBE

S Pombe 中细胞分裂平面的放置机制

• 批准号: 6056019
• 负责人: PHONG T TRAN
• 金额: $ 3.24万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6056019
• 关键词: Schizosaccharomyces pombe; cell component structure /function; cell cycle; cell nucleus; digital imaging; fungal genetics; gene targeting; lasers; microorganism culture; microtubules; protoplasm motility

Cell division is a fundamental cellular process about which little is known at the molecular level. Defects in cell division may lead to cancer and other diseases. The aim of this proposal is to use a combination of molecular genetics and biophysical approaches to answer the question: How does a cell know where to divide? The model of choice for this proposal is S. pombe, a single-cell eukaryote with simple cytoskeletal architecture, powerful genetics, and amenability for live cell imaging. In S. pombe the position of the division plane appears to be dictated by the position of the nucleus. Furthermore, nuclear positioning at the cell center is microtubule dependent. This proposal aims to use biophysical (e.g., UV ablation, high-resolution multi-mode digital microscopy, laser cutting, etc ...) and molecular genetic (e.g., gene knockout, mutant phenotype analysis, GFP-tagging, etc ...) approaches to: I) determine how the nucleus and/or the microtubules may produce forces that position the nucleus; (II) determine the function of a novel S. pombe gene, rsp1, that participates in microtubule organization and nuclear positioning.

细胞分裂是一个基本的细胞过程，围绕分子水平知之甚少。 细胞分裂的缺陷可能导致癌症和其他疾病。 该提案的目的是利用分子遗传学和生物物理方法的组合来回答以下问题：细胞如何知道在哪里分裂？ 该提案的首选模型是S. Pombe，它是一种具有简单细胞骨架结构，强大遗传学和实时细胞成像的单细胞真核生物。 在S. pombe中，分裂平面的位置似乎由核的位置决定。 此外，细胞中心的核定位是微管依赖性的。该建议旨在使用生物物理（例如，紫外线消融，高分辨率多模式数字显微镜，激光切割等）和分子遗传（例如，基因敲除，突变表型分析，GFP tagggging等... （ii）确定参与微管组织和核定位的新型pombe基因RSP1的功能。