AUTOMATED MONITORING OF CHEMOTACTIC CELL MOVEMENT

自动监测趋化细胞运动

• 批准号: 6054380
• 负责人: MICHAEL A LYNES
• 金额: $ 10万
• 依托单位: APPLIED BIOPHYSICS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2001-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6054380
• 关键词: biomedical automation; biomedical equipment development; cell migration; chemotaxis; electrical impedance; electrodes; high throughput technology; monitoring device

Our phase I objective is to manufacture and test a series of instrument designs that will be capable of automated real time assessment of chemotactic cell movement in culture. Ultimately, we will design systems capable of high throughput screening for chemokines and other cell attractants. Moreover, these systems will facilitate automated assessment of pharmaceuticals that mediate or moderate chemotaxis, as well as drugs that modify non-directional movement (chemokinesis) that can be associated with cancer metastasis. When these objectives have been successfully met, the instrument will be invaluable to pharmaceutical and biotechnology firms that are interestedin manipulation of cell motility that is associated with (for example) inflammation, autoimmune disease, and neoplasia. This work represents and entirely novel approach to the automated measurement of cell movement. PROPOSED COMMERCIAL APPLICATION: The instrument to be developed will enable scientists and clinicians to measure cell movement responses to drugs and other pharmaceuticals. This will vastly improve our assessment of specific disease states (e.g. inflammation, autoimmune disease, and neoplasia) and speed the development of therapeutic drugs for these conditions. The instrument will also facilitate the identification of gene disruptions that impede chemotactic cell responses, enabling the identification of genes that regulate the innate capacity of a cell to respond to external signals.

我们的I阶段目标是制造和测试一系列仪器设计，这些仪器设计能够对培养物中趋化细胞运动的实时评估自动化。最终，我们将设计能够对趋化因子和其他细胞吸引剂进行高吞吐量筛选的系统。此外，这些系统将促进对介导或中度趋化性的药物的自动评估，以及可以改变与癌症转移有关的非方向运动（趋化因子）的药物。 当这些目标成功实现时，该仪器将对与（例如）炎症，自身免疫性疾病和肿瘤有关的细胞运动感兴趣的药物和生物技术公司无价。 这项工作代表了自动测量细胞运动的方法，并且完全是新颖的方法。拟议的商业应用：要开发的工具将使科学家和临床医生能够测量对药物和其他药物的细胞运动反应。这将大大改善我们对特定疾病状态（例如炎症，自身免疫性疾病和肿瘤）的评估，并加快了这些疾病治疗药物的发展。 该仪器还将促进鉴定阻碍趋化细胞反应的基因破坏，从而鉴定基因调节细胞对外部信号的响应。