SORTING AND ASSEMBLY OF THE HSV-1 TEGUMENT PROTEIN VHS

HSV-1 外皮蛋白 VHS 的分类和组装

• 批准号: 6150368
• 负责人: DUNCAN W. WILSON
• 金额: $ 17.55万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6150368
• 关键词: Golgi apparatus; cell line; cell membrane; chimeric proteins; confocal scanning microscopy; green fluorescent proteins; herpes simplex virus 1; immunocytochemistry; immunoelectron microscopy; immunoprecipitation; intracellular transport; laboratory rabbit; organelles; protein signal sequence; protein structure function; site directed mutagenesis; virion; virus assembly; virus envelope; virus protein; western blottings

The Herpes viruses are responsible for a large number of human diseases. All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of the viral envelope. Correct assembly of tegument and envelope is essential for the production of an infectious virus particle, and thus for the progression of disease. However, little is known of how tegument proteins recognize and bind to specific cellular membranes, nor how they ensure their assembly into the maturing virion. This proposal will investigate membrane and tegument sorting information present within the Herpes simplex virus (HSV) tegument protein vhs. We will identify the cytoplasmic organelle which binds vhs, and determine the role of this association in the HSV life cycle. Sequences responsible for vhs/organelle binding will be identified, and we will determine which regions of vhs ensure incorporation of this protein into the mature virus. Information obtained from this study will help in the design of agents able to interfere with vhs assembly into the HSV particle, reducing the virulence of this serious human pathogen.

疱疹病毒导致大量人类疾病。 所有疱疹病毒都有一个称为tegument的蛋白质层 线的内部表面。 正确的组装 Tegument和信封对于产生传染性至关重要 病毒粒子，因此是疾病的进展。 然而， 鲜为人知的蛋白质如何识别并结合特定 细胞膜，或者如何确保其组装到成熟 病毒。 该建议将调查膜和Tegument分类信息 存在于单纯疱疹病毒（HSV）Tegument蛋白VHS中。 我们 将识别结合VHS的细胞质细胞器，并确定 这种关联在HSV生命周期中的作用。 序列 将确定负责VHS/Organelle绑定，我们将 确定VHS的哪些区域确保将该蛋白掺入 成熟的病毒。 从这项研究获得的信息将有助于 能够干扰VHS组件进入HSV的代理的设计 粒子，降低了这种严重的人类病原体的毒力。