Biochemistry of Herpes Simplex Tegument Components

单纯疱疹外皮成分的生物化学

• 批准号: 7090988
• 负责人: DUNCAN W. WILSON
• 金额: $ 28.89万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2007-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7090988
• 关键词: Alphaherpesvirinae; Herpes simplex disease; biochemistry; host organism interaction; microorganism culture; protein binding; protein purification; protein structure function; virion; virus assembly; virus envelope; virus protein

The Herpes viruses are responsible for many human diseases. All Herpes viruses possess a proteinaceous layer termed tegument which lines the inner surface of their envelope. Correct assembly of tegument and envelope is essential for the production of an infectious viral particle, and thus for the progression of disease. However, little is known of how tegument proteins recognize and bind to specific cellular membranes, nor how they ensure their assembly into the maturing virion. The first part of this proposal will investigate the biochemistry of the Herpes simplex virus (HSV) protein vhs, a component of the HSV tegument. We have previously identified several biochemically distinct forms of vhs in infected cells. We will now investigate the relationship between these molecules and determine their importance for assembly of vhs into tegument. Next, we will dissect the molecular mechanism by which vhs binds to intracellular membranes. Finally, we have successfully isolated primary enveloped HSV particles from the perinuclear space. We will study the composition and function of tegument in these HSV assembly intermediates.

疱疹病毒导致许多人类疾病。所有的疱疹病毒都具有称为tegument的蛋白质层，该层将其信封的内表面排列。正确组装Tegument和Invelope对于产生传染性病毒颗粒以及疾病进展至关重要。然而，几乎不知道tegument蛋白如何识别并结合特定的细胞膜，也不知道它们如何确保它们的组装到成熟的病毒体中。该提案的第一部分将研究HSV Tegument的组成部分的单纯疱疹病毒（HSV）蛋白质VHS的生物化学。我们以前有 在感染细胞中鉴定出了几种生化不同形式的VHS。现在，我们将研究这些分子之间的关系，并确定它们对VHS组装到Tegument中的重要性。接下来，我们将剖析VHS与细胞内结合的分子机制 膜。最后，我们从与 核周空间。我们将研究Tegument在这些HSV组装中间体中的组成和功能。