THE ROLE OF LYT1 PROTEIN IN TRYPANOSOMA CRUZI

LYT1 蛋白在克氏锥虫中的作用

• 批准号: 6053350
• 负责人: John T. Swindle
• 金额: $ 44.14万
• 依托单位: ACCESS TO ADVANCED HEALTH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-07 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053350
• 关键词: Trypanosoma cruzi; alleles; arthropod borne communicable disease; gene expression; genetic manipulation; host organism interaction; intracellular parasitism; mass spectrometry; microarray technology; microorganism growth; nucleic acid probes; pathologic process; phenotype; polymerase chain reaction; posttranscriptional RNA processing; protein protein interaction; protein structure function; protozoal infection; southern blotting; trypanosomiasis; yeast two hybrid system

DESCRIPTION (Adapted from the Applicant's Abstract): The objective of this work is to dissect the role of protein LYT1p in T. cruzi development and pathogenesis. This protein was first identified in the lab of Antonio Gonzalez (Spain) who found it by screening a cDNA library with antibody directed to the mammalian C9 component of complement. LYT1 is a single copy gene that is dispensable in T. cruzi epimastigotes. However, LYT1 deficient parasites have three phenotypes: 1) they are infection deficient; 2) they exhibit accelerated in vitro development; and 3) they have diminished hemolytic activity. There are two main goals in this project: 1) to understand how the LYT1p is involved in the biological processes leading to human infection; and 2) to use LYT1p deficient parasites to identify stage specific genes involved in parasite development.

描述（改编自申请人的摘要）：这项工作的目的 是为了剖析蛋白质lyt1p在克鲁兹开发中的作用和 发病。该蛋白质最初是在安东尼奥·冈萨雷斯（Antonio Gonzalez）实验室中鉴定出来的 （西班牙）通过筛选针对抗体的cDNA库来找到它 补体的哺乳动物C9成分。 lyt1是一个复制基因 在克鲁兹t. ipimastigotes中可分配。但是，Lyt1缺乏的寄生虫具有 三种表型：1）它们是感染不足的； 2）他们表现出加速 体外发展； 3）它们减少了溶血活性。有 该项目的两个主要目标：1）了解LYT1P如何参与 导致人类感染的生物学过程； 2）使用lyt1p 缺乏寄生虫以识别寄生虫涉及的特定阶段特定基因 发展。