Genetics of Dengue Vector Competence in Aedes aegypti

埃及伊蚊登革热媒介能力的遗传学

• 批准号: 6710088
• 负责人: WILLIAM C. BLACK
• 金额: $ 28.64万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6710088
• 关键词: Aedes; alleles; animal population genetics; arthropod borne communicable disease; communicable disease transmission; dengue; dengue virus; disease vectors; epizootiology; gene expression; gene frequency; genetic mapping; genetic markers; genetic polymorphism; genetic strain; genetic susceptibility; genotype; host organism interaction; molecular cloning; quantitative trait loci; serotyping; subtraction hybridization

DESCRIPTION (provided by applicant): Dengue fever is one of the most rapidly expanding diseases in the tropics with over 2 billion people at risk. Transmission of Dengue virus (DEN) involves a complex interaction between the genomes of the primary mosquito vector, Aedes aegypti, the virus, and its human host. The proposed research will focus on two of these components by examiningthe genetics of vector competence in Ae. aegypti and the degree to which vector competence is affected by genetic variance in the DEN virus. We have mapped Quantitative Trait Loci (QTL) affecting the ability of Ae. aegypri midgut to become infected with Dengue-2 virus (DEN2). These twomidgut infection barrier (MIB) QTL are called dmib2, dmib3 and were mapped to chromosomes II and III respectively. The primary goal of the proposed work is to use marker-assisted selection (MAS) to generate 4 strains of Ae. aegypri. These will be a strain homozygous susceptible at both loci (DS2), a strain homozygous refractory at both loci (DR2) and 2 strains homozygous susceptible at one locus and homozygous refractory at the other locus (DMIB2, DMIB3). These strains will provide an exceptional opportunity to elucidate the phenotypes associated with each genotype at the QTL underlying DEN transmission. DS2 will be used to isolate and map DEN barrier QTL from worldwide populations of Ae. aegypri to determine if additional QTL control DEN susceptibility and/or if alternate alleles exist at the currently identified QTL. DMIB2, DMIB3, and DR2 will be used to assess how alleles at both loci separately and together confer susceptibility in different DEN serotypes and genotypes in each of the 4 serotypes. DS2 and DR2 will be used in the construction of recombinant inbred lines for use in a subtractive hybridization approach to clone cDNAs putatively encoded bydmib3and to assist in a collaborative effort to positionally clone dmib3.

描述（由申请人提供）：登革热是传播最快的疾病之一 热带地区疾病不断蔓延，超过 20 亿人面临风险。 登革热病毒 (DEN) 的传播涉及病毒之间复杂的相互作用 主要蚊子媒介埃及伊蚊、该病毒及其人类的基因组 主持人。拟议的研究将重点关注其中两个组成部分 检查伊蚊载体能力的遗传学。埃及伊蚊和程度 该载体的能力受 DEN 病毒遗传变异的影响。我们 绘制了影响 Ae 能力的数量性状位点 (QTL)。埃及伊蚊 中肠感染登革热 2 病毒 (DEN2)。这两种中肠感染 屏障 (MIB) QTL 称为 dmib2、dmib3，被定位到染色体 II 和 三、分别。拟议工作的主要目标是使用 标记辅助选择（MAS）产生 4 株伊蚊。伊吉普里。这些 将是在两个基因座（DS2）上均易感的纯合菌株，纯合菌株 两个位点均难治（DR2），2 个菌株在一个位点纯合易感 以及其他基因座（DMIB2、DMIB3）的纯合难治性。这些菌株将 提供了一个难得的机会来阐明与相关的表型 DEN 传播基础 QTL 处的每个基因型。 DS2 将用于 从世界各地的伊蚊种群中分离并绘制 DEN 屏障 QTL。伊吉普里托 确定额外的 QTL 是否控制 DEN 易感性​​和/或是否替代 等位基因存在于当前确定的 QTL 处。 DMIB2、DMIB3 和 DR2 将是 用于评估两个基因座上的等位基因分别和一起如何赋予 4种不同DEN血清型和基因型的易感性 血清型。 DS2和DR2将用于构建重组近交系 用于通过消减杂交方法克隆 cDNA 的品系 由 dmib3 编码并协助协作进行定位克隆 dmib3。