ANTI-HIV-1 ACTIVITY OF MANNAN-BINDING LECTIN

甘露聚糖结合凝集素的抗 HIV-1 活性

• 批准号: 6213956
• 负责人: GREGORY T SPEAR
• 金额: $ 25.03万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6213956
• 关键词: antiviral antibody; glycosylation; human immunodeficiency virus 1; human subject; lectin; mannans; physical chemical interaction

DESCRIPTION: (Adapted from Abstract) Mannan-binding lectin (MBL) is an effector molecule of the innate immune system found in human serum that binds to carbohydrate and mediates recognition and killing of pathogens. Although the human immunodeficiency virus (HIV) would appear to be a good target of MBL due the unusually high level of high mannose glycosylation of gpl20, this has not been well studied. Our research - group found that MBL binds to a wide range of HIV isolates including primary isolates. Thus, MBL has several highly desirable features of an anti-HIV effector molecule; 1) it binds to a wide range of virus isolates; 2) binding of MBL to HIV is likely via the unusual cluster of high mannose microgram levels. These features of MBL suggest it mediates anti-viral activity in vivo and inforrnation gained about its interaction with HIV will lead to a better understanding of both the role of innate immunity during HIV infection and the importance of carbohydrate structures on HIV. The overall goals of this study are to determine the mechanism of interaction between MBL and HIV and to determine methods to enhance the anti-HIV activity of MBL. These goals will be accomplished by the following specific aims: Deterrnine the type and location of carbobydrates responsible for high-level binding of HIV- 1 to MBL. 2. Investigate factors that affect the interaction between HIV and MBL. 3. Utilize inhibitors of glycosylation to enhance biological effects of MBL. Exarnine the interaction of MBL with anti-viral antibodies in mediating anti-viral effects. Deterrnine the interaction of HIV-1 with MBL in vivo.

描述：（改编自摘要）Mannan结合凝集素（MBL）是效应器 在人血清中发现的先天免疫系统的分子，与 碳水化合物并介导病原体的识别和杀死。虽然 人类免疫缺陷病毒（HIV）似乎是MBL的良好目标 GPL20的高甘露糖糖基化非常高的高水平，这还没有 经过深入研究。我们的研究 - 小组发现，MBL结合了广泛的 HIV分离株，包括主要分离株。因此，MBL具有多个非常理想的 抗HIV效应分子的特征； 1）它与多种病毒结合 分离株； 2）MBL与HIV的结合可能是通过不寻常的高簇 mannose微克水平。 MBL的这些特征表明它介导了抗病毒 在体内的活性和浮肿，就其与HIV的相互作用而获得了 可以更好地理解艾滋病毒中先天免疫的作用 感染和碳水化合物结构在HIV上的重要性。总体 这项研究的目标是确定MBL之间相互作用的机制 HIV并确定增强MBL抗HIV活性的方法。这些 目标将通过以下特定目的来实现：类型的威慑 和负责HIV-1高水平结合的碳纤维材料的位置 MBL。 2。研究影响HIV与MBL之间相互作用的因素。 3。 利用糖基化的抑制剂来增强MBL的生物学作用。 Exarnine MBL与抗病毒抗体的相互作用 抗病毒作用。威慑HIV-1与体内MBL的相互作用。