STRUCTURAL BASIS OF T CELL RECEPTOR RECOGNITION

T 细胞受体识别的结构基础

• 批准号: 6137238
• 负责人: IAN A WILSON
• 金额: $ 22.3万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6137238
• 关键词: CD3 molecule; CD8 molecule; MHC class I antigen; T cell receptor; T lymphocyte; X ray crystallography; antigen presentation; biological signal transduction; molecular cloning; protein structure function; tissue /cell culture

DESCRIPTION (Adapted from Investigator's abstract): The goal of this proposal is to answer a number of fundamental questions regarding the structural basis for the recognition of peptide/MHC class I complexes by T cell receptors (TCRs). Are there general structural principles which govern TCR-MHC interactions? Are these similar for TCR recognition of self and foreign MHC? Does conformational change or induced fit play a role in TCR-MHC recognition and communication among members of the TCR/MHC/CD3/CD8 signalling complex? X-ray crystallography will be used to investigate the detailed molecular interactions between a TCR specific for different syngeneic and allogeneic peptide/MHC class I complexes in the presence and absence of CD3 and CD8 components. The specific aims are to determine the structures of: (i) TCR-MHC syngeneic class I-peptide complexes, (ii) TCR-allogeneic class I-peptide complexes, (iii) TCR/MHC/CD8 ternary complexes, (iv) agonist and antagonist TCR-MHC complexes, and (v) extracellular CD3 chains and their complexes with TCR.

描述（改编自研究者的摘要）：本研究的目标 提案旨在回答有关 T 识别肽/MHC I 类复合物的结构基础 细胞受体（TCR）。 是否有通用的结构原则来指导 TCR-MHC 相互作用？ 这些对于自我和 TCR 识别是否相似？ 国外MHC？ 构象变化或诱导契合是否在其中发挥作用？ TCR/MHC/CD3/CD8成员之间的TCR-MHC识别和交流 信号复合体？ X射线晶体学将用于研究 不同TCR特异性的详细分子相互作用 同基因和同种异体肽/MHC I 类复合物的存在和 缺乏 CD3 和 CD8 成分。 具体目标是确定 (i) TCR-MHC 同源 I 类肽复合物的结构，(ii) TCR-同种异体 I 类肽复合物，(iii) TCR/MHC/CD8 三元 复合物，(iv) 激动剂和拮抗剂 TCR-MHC 复合物，以及 (v) 细胞外 CD3 链及其与 TCR 的复合物。