NMR studies of viral proteins

病毒蛋白的核磁共振研究

• 批准号: 6340733
• 负责人: CAROL B. POST
• 金额: $ 16.62万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6340733
• 关键词: Flaviviridae; RNA virus; Rubivirus; bioimaging /biomedical imaging; capsid; hepatitis C virus; nuclear magnetic resonance spectroscopy; nucleocapsid; protein protein interaction; protein structure function; structural biology; virus assembly; virus protein; virus replication

Structural and biochemical studies are proposed to examine protein- protein and protein-lipid interactions involved in viral budding and viral maturation. The focus is on the plus-strand RNA viruses, which either cause human disease or are closely related to viruses which cause human disease. The budding process and nucleocapsid stabilization will be investigated by NMR and other methods for alphavirus, hepatitis C virus, rubella virus, and yellow fever virus, the most simple enveloped viruses. Structural features important for capsid stabilization and maturation will be investigated by NMR for Rous sarcoma virus (RSV), a structurally more complex retrovirus. Specific objectives are to understand the importance of the capsid-nucleocapsid region of the RSV Gag protein by determining 3-dimensional solution structures of various RSV protein constructs, and characterizing interdomain interactions for the C-terminal domain of capsid (CA) protein, the nucleocapsid (NC) protein, and the capsid-nucleocapsid core particles and the transmembrane E2- glycoprotein has been proposed and will be tested by biochemical and NMR experiments. Further studies to investigate nucleocapsid stability are also proposed for yellow fever virus (YFV), hepatitis C virus (HCV), and rubella virus (RUBV) using NMR methods combined with mutagenesis results. Results from the studies proposed in this project, coupled with results from molecular genetics and crystallography proposed elsewhere in the Program Project, will enhance our understanding of the structural and physical basis underlying viral assembly.

提出了结构和生化研究，以检查参与病毒萌芽和病毒成熟的蛋白质蛋白质和蛋白质脂质相互作用。重点放在链链RNA病毒上，该病毒会引起人类疾病或与引起人类疾病的病毒密切相关。 NMR将研究出芽孢杆菌，丙型肝炎病毒，风疹病毒和黄热病病毒的其他方法，这是最简单的包围病毒。 NMR将研究对衣壳稳定和成熟重要的结构特征。 Specific objectives are to understand the importance of the capsid-nucleocapsid region of the RSV Gag protein by determining 3-dimensional solution structures of various RSV protein constructs, and characterizing interdomain interactions for the C-terminal domain of capsid (CA) protein, the nucleocapsid (NC) protein, and the capsid-nucleocapsid core particles and the transmembrane E2-已经提出了糖蛋白，并将通过生化和NMR实验进行测试。还提出了使用NMR方法与诱变结果结合使用的黄热病病毒（YFV），丙型肝炎病毒（HCV）和红宝石病毒（RUBV）的进一步研究来研究Nucleocapsid稳定性。该项目中提出的研究的结果，再加上计划项目其他地方提出的分子遗传学和晶体学的结果，将增强我们对结构和物理基础病毒组装的理解。