PROTEIN INTERACTIONS AND REPRESSION FUNCTION OF E1A

E1A 的蛋白质相互作用和抑制功能

基本信息

批准号：
6172484
负责人：
Elizabeth Moran
金额：
$ 33.17万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-08-01 至 2005-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6172484
关键词：
Adenoviridae DNA binding protein enzyme activity gene expression helicase intermolecular interaction molecular cloning protein structure function tissue /cell culture transcription factor virus protein

项目摘要

DESCRIPTION: The E1A-associated protein, p300, is of major interest to cancer biologists because, as a direct target of the transforming functions of the adenovirus E1A gene, it is implicated in the regulation of both cell cycle specific and tissue specific gene expression. p300 is highly homologous to the CREB coactivator, CBP. CREB and CBP are the major mediators of the transcriptional response to cyclic AMP-responsive pathways. The relationship between p300 and CBP suggests that they perform, similar functions. Nevertheless, they are encoded by different genes, and preliminary clinical evidence indicates that specific metabolic disorders are linked with selective loss of CBP, indicating that p300 and CBP are not functionally redundant. The investigators identified a new 270 kilodalton product that is related to both p300 and CBP. p270 is particularly interesting because it exists in cells as a component of a tightly associated series of proteins. Moreover they have identified a prominent member of this complex as BRG1. The BRG1 protein product is a transcriptional activator, a regulator of gene expression pathways linked with the development and differentiation, and a putative DNA helicase. The p207/BRG1 complexes with this activity intact. The identification of this interaction should open the way to understanding a major part of the molecular mechanisms of action of the p300 and BRG1 families, two families of proteins that appear to be pivotal regulators of gene expression in pathways controlling cell proliferation and differentiation.

描述：E1A相关蛋白P300是主要的兴趣癌症生物学家是因为，作为转化功能的直接目标腺病毒E1a基因的调节与两个细胞的调节有关循环特异性和组织特异性基因表达。 P300高度与CREB共激活因子CBP同源。 Creb和CBP是主要的对环状AMP响应途径的转录响应的介体。 P300和CBP之间的关系表明它们的性能类似功能。然而，它们是由不同基因编码的，初步临床证据表明特定的代谢性疾病与CBP的选择性丢失有关，表明P300和CBP不是在功能上冗余。调查人员确定了一个新的270千达尔顿与P300和CBP相关的产品。 P270尤其是有趣，因为它存在于细胞中，作为紧密的组成部分相关系列蛋白质。而且他们已经确定了一个突出的该复合物的成员为BRG1。 BRG1蛋白产品是转录激活剂，基因表达途径的调节剂随着开发和分化以及推定的DNA解旋酶。这 P207/BRG1复合物具有完整的。识别此互动应该为理解主要部分 p300和BRG1家族的作用分子机制，两个家族似乎是基因表达的关键调节剂的蛋白质控制细胞增殖和分化的途径。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The DNA-binding properties of the ARID-containing subunits of yeast and mammalian SWI/SNF complexes.

DOI：
10.1093/nar/gkh277
发表时间：
2004-02
期刊：
Nucleic acids research
影响因子：
14.9
作者：
D. Wilsker;A. Patsialou;Steven D. Zumbrun;Suhkmann Kim;Yuan Chen;P. Dallas;E. Moran
通讯作者：
D. Wilsker;A. Patsialou;Steven D. Zumbrun;Suhkmann Kim;Yuan Chen;P. Dallas;E. Moran

DNA-binding properties of ARID family proteins.

DOI：
10.1093/nar/gki145
发表时间：
2005
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Patsialou A;Wilsker D;Moran E
通讯作者：
Moran E

ARID proteins: a diverse family of DNA binding proteins implicated in the control of cell growth, differentiation, and development.

DOI：
发表时间：
2002-03
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子：
0
作者：
D. Wilsker;A. Patsialou;P. Dallas;E. Moran
通讯作者：
D. Wilsker;A. Patsialou;P. Dallas;E. Moran

Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP.

单克隆抗体 NM11 可识别 p300 和 CBP 共享的 C 末端表位。