Functional Genomics of Human Brain Development Cluster

人脑发育集群的功能基因组学

• 批准号: MR/Y031016/1
• 负责人: Oscar Marin
• 金额: $ 534.52万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FY031016%2F1
• 关键词: Functional; Genomics; Human; Brain; Development

Neurodevelopmental conditions like autism, attention deficit hyperactivity disorder (ADHD), epilepsy, and intellectual disability affect millions of children worldwide. These conditions are highly complex, and their causes remain elusive. Recent technological advances have revealed that genetics play a crucial role in these disorders. However, we still need to understand how specific gene variations disrupt the development of the human brain and lead to alterations that disrupt the lives of affected individuals. Our goal is to unlock the secrets behind neurodevelopmental disorders and find ways to help affected children and their families lead better lives.Our team of scientists will be using brain organoids, three-dimensional "avatars" of the human brain grown in the lab from human stem cells, to study the developing brain in a more accurate and detailed way. These organoids mimic some of the cellular complexity of the human brain better than previous methods, allowing us to study how genes influence brain development and function. We will initially focus on the cerebral cortex, a critical brain region responsible for higher functions like planning and memory. By studying brain organoids containing different types of brain cells, we aim to discover the role of specific genes associated with neurodevelopmental disorders. This research will help us identify the causes of these conditions and pave the way for developing targeted treatments.One significant challenge is that brain organoids created in different labs can vary, affecting the reliability of the research. To overcome this, we will create a standardised platform for generating brain organoids, ensuring consistency and reproducibility across multiple research centres in the UK. We will also use cutting-edge technologies like single-cell genomics, imaging, and electrophysiology to analyse brain organoids and unravel the intricate pathways contributing to human brain development in health and disease. We will also investigate how different people's genetic backgrounds influence these disorders, ensuring our findings are representative and applicable to diverse populations.We will bring together scientists from eight institutions in the UK and worldwide. Team members from these institutions are already collaborating by sharing resources and data. By developing this platform, we can extend these interactions and combine their expertise to share our findings and resources nationally. This approach will multiply our collective capacity to investigate the molecular mechanisms regulating brain development and their dysregulation in disease. We will accelerate the ability of other researchers to carry out investigations using brain organoids through dedicated training, sharing of protocols, and outreach programs in which we will interact with researchers and the general community, facilitating effective knowledge-sharing across multiple groups of individuals.In summary, this collaboration will accelerate our understanding of the biology underlying human brain development and the role that specific gene changes play in acquiring a neurodevelopmental condition. We envision that this knowledge will ultimately help identify new ways to improve the lives of children and families affected by these conditions.

自闭症、注意力缺陷多动障碍 (ADHD)、癫痫和智力障碍等神经发育疾病影响着全世界数百万儿童。这些情况非常复杂，其原因仍然难以捉摸。最近的技术进步表明，遗传学在这些疾病中发挥着至关重要的作用。然而，我们仍然需要了解特定的基因变异如何扰乱人类大脑的发育并导致改变，从而扰乱受影响个体的生活。我们的目标是解开神经发育障碍背后的秘密，并找到帮助受影响的儿童及其家人过上更好生活的方法。我们的科学家团队将使用大脑类器官，即在实验室中从人类身上培育出来的人脑的三维“化身”干细胞，以更准确和详细的方式研究发育中的大脑。这些类器官比以前的方法更好地模拟了人脑的一些细胞复杂性，使我们能够研究基因如何影响大脑的发育和功能。我们首先将重点关注大脑皮层，这是负责计划和记忆等高级功能的关键大脑区域。通过研究含有不同类型脑细胞的大脑类器官，我们的目标是发现与神经发育障碍相关的特定基因的作用。这项研究将帮助我们确定这些病症的原因，并为开发针对性治疗铺平道路。一个重大挑战是，不同实验室创建的大脑类器官可能会有所不同，从而影响研究的可靠性。为了克服这个问题，我们将创建一个标准化平台来生成大脑类器官，确保英国多个研究中心的一致性和可重复性。我们还将利用单细胞基因组学、成像和电生理学等尖端技术来分析大脑类器官，并揭示在健康和疾病中促进人类大脑发育的复杂途径。我们还将调查不同人的遗传背景如何影响这些疾病，确保我们的研究结果具有代表性并适用于不同的人群。我们将汇集来自英国和世界各地八个机构的科学家。来自这些机构的团队成员已经通过共享资源和数据进行协作。通过开发这个平台，我们可以扩展这些互动并结合他们的专业知识，在全国范围内分享我们的发现和资源。这种方法将增强我们研究调节大脑发育及其在疾病中失调的分子机制的集体能力。我们将通过专门的培训、协议共享和外展计划，加快其他研究人员使用脑类器官进行研究的能力，在这些计划中，我们将与研究人员和广大社区互动，促进多个群体之间的有效知识共享。总之，这项合作将加速我们对人类大脑发育背后的生物学以及特定基因变化在获得神经发育状况中所起的作用的理解。我们预计，这些知识最终将有助于找到新的方法来改善受这些条件影响的儿童和家庭的生活。