WOMENS HEALTH ACROSS THE NATION--ENDOCRINE LAB

全国女性健康--内分泌实验室

• 批准号: 6124102
• 负责人: Alvin Rees Midgley
• 金额: $ 117.37万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6124102
• 关键词: aging; analytical chemistry; analytical method; biomarker; clinical chemistry; clinical research; cooperative study; dehydroepiandrosterone; estradiol; estrone; female; follicle stimulating hormone; human middle age (35-64); human tissue; immunologic assay /test; inhibin; luteinizing hormone; menopause; method development; progesterone; sex hormones; testosterone; thyrotropin; tissue resource /registry; women's health

To assess endocrine changes in midlife women, the University of Michigan's Central Ligand Assay Satellite Services (CLASS) laboratory will continue to support the Study of Women's Health Across the Nation (SWAN). We will use state-of-the-science, automated assays for all the major reproductive axis hormones (i.e., LH, FSH, estradiol, progesterone, and testosterone), adrenal markers of aging (DHEAS), other endocrine markers (TSH, sex hormone binding globulin {SHBG}) and new ovarian markers which have the potential to allow us to hormonally define the menopausal transition and the postmenopause with greater precision (inhibin A and B, estrone). In order to continue to support the Daily Hormone Study (DHS), CLASS will complete development and utilize sensitive and specific urinary assays for LH, FSH and the principal urinary metabolites of estradiol and progesterone, estrone conjugates and pregnanediol glucuronide, respectively. Our specific goals for the proposed project period are to: 1) utilize, implement and develop state-of-the-science, reliable, precise and cost-effective analytical methods that meet all study needs (analyte selection, limits of detection, range of performance), minimize variability, provide long-term stability and maximize reliability; 2) incorporate quality assurance procedures that minimize error and variability as assessed by rigorous quality control procedures; 3) utilize and develop additional electronic data handling and analysis procedures that, in addition to reducing labor costs, minimize the need for manual intervention and nearly eliminate the possibility of errors; 4) utilize sample labeling and handling systems that ensure continuous association between a labeled sample and data identified with the tube while maximizing the likelihood that the integrity of a sample is preserved unmodified; and 5) develop a temporary storage repository. In this manner, we will continue to play a key role in the advancement of the scientific goals of the parent SWAN study.

为了评估中年妇女的内分泌变化，密歇根大学的中央配体分析卫星服务（班级）实验室将继续支持全国妇女健康的研究（SWAN）。 We will use state-of-the-science, automated assays for all the major reproductive axis hormones (i.e., LH, FSH, estradiol, progesterone, and testosterone), adrenal markers of aging (DHEAS), other endocrine markers (TSH, sex hormone binding globulin {SHBG}) and new ovarian markers which have the potential to allow us to hormonally define the更年期过渡和绝经后的精确度更高（抑制素A和B，雌酮）。 为了继续支持每日激素研究（DHS），类别将完成发育，并分别对LH，FSH和雌二醇和孕酮的主要尿尿代谢产物，分别为LH，FSH和特定的尿液分析。 我们针对拟议项目期间的具体目标是：1）利用，实施和发展水平，可靠，准确且具有成本效益的分析方法，满足所有研究需求（分析物选择，检测范围，绩效范围），最小化可变性，提供长期稳定性并最大程度地提高可靠性； 2）合并质量保证程序，以最大程度地减少严格质量控制程序评估的错误和可变性； 3）利用和开发其他电子数据处理和分析程序，除了降低人工成本外，还可以最大程度地减少手动干预的需求，并几乎消除了错误的可能性； 4）利用样品标记和处理系统，以确保标记的样品和与管子识别的数据之间的连续关联，同时最大程度地保留样品的完整性未修饰的可能性； 5）开发一个临时存储存储库。 通过这种方式，我们将继续在父母天鹅研究的科学目标的发展中发挥关键作用。