Imaging Life with Light and Sound: State-of-the-art Photoacoustic Imaging For Mechanistic Understanding Of Human Disease

用光和声音成像生命：最先进的光声成像用于理解人类疾病的机制

• 批准号: MR/X012549/1
• 负责人: Maya Thanou
• 金额: $ 69.91万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX012549%2F1
• 关键词: Imaging; Life; Light; Sound; State

Photoacoustic imaging (PAI) involves shining a particular type of light into the body and then detecting sound waves coming out of the body. This can provide pictures showing the structure of organs, how they are working, and what certain molecules are doing in the body. PAI is safe and can look relatively deep into the body. It is important to be able to study how organs work in the body, what is happening in disease, in a living subject, rather than "in a dish" which does not reflect so well what happens in the body.We will use the most advanced PAI system commercially available to study heart, cardiovascular and brain disease and cancer in experimental models. PAI can be used to study organs such as the heart and the brain in living subjects, to see if organs are working properly, e,g., is the heart beating properly, is a blood vessel blocked, is blood flowing properly in the brain, is enough oxygen being delivered to the brain, etc. We can also use PAI to see if there is disease anywhere in the body, e,g., cancer. PAI can be used to see particular molecules that are changed when there is disease, e.g., the abnormal protein clumps in the brain in Alzheimer's and Parkinson's Disease. Sometimes molecules have to be tagged to be seen by PAI, and in this project, we will invent new tags/probes to see molecules we think are involved in causing disease. Disease can also be caused by certain cells not working properly and we can also tag these cells so that we can use PAI to see where they are and what they are doing. Sometimes drugs are delivered by specially made tiny particles or even in safe virus particles, and these can also be tagged so that we can see where drugs are being delivered by PAI. Using PAI we will get detailed pictures of what happens during human disease so that we can identify harmful molecules or cells that are important in causing disease and develop new drugs that will target these molecules and cells.

光声成像（PAI）涉及将特定类型的光线照射到体内，然后检测到体内的声波。这可以提供显示器官结构，它们的工作方式以及某些分子在体内所做的事情的图片。 PAI是安全的，可以看起来相对深入体内。重要的是要研究器官如何在体内工作，疾病，生命主题中正在发生的事情，而不是“在菜肴中”，这并不能很好地反映身体中发生的事情。我们将在实验模型中使用商业上最先进的PAI系统来研究心脏，心血管和脑病和癌症。 PAI可用于研究生物中的心脏和大脑等器官，看看器官是否正常工作，e，g。的心脏是否正常跳动，是一种血管，是一种血管阻塞，血液在大脑中正确流动，足以将氧气传递到大脑中。 PAI可用于查看疾病时发生变化的特定分子，例如，阿尔茨海默氏病和帕金森氏病的大脑中异常的蛋白质团块。有时，必须将分子标记为PAI，在这个项目中，我们将发明新的标签/探针，以查看我们认为引起疾病的分子。疾病也可能是由于某些细胞无法正常工作而引起的，我们也可以标记这些细胞，以便我们可以使用PAI查看它们的位置以及它们在做什么。有时，药物是由专门制成的微粒甚至是安全的病毒颗粒传递的，并且可以标记这些药物，以便我们可以看到PAI递送药物的位置。使用PAI，我们将获得有关人类疾病期间发生的情况的详细图片，以便我们可以鉴定有害分子或细胞，这些分子或细胞在引起疾病和开发针对这些分子和细胞的新药物。

项目成果

Cyclin D-CDK4 Disulfide Bond Attenuates Pulmonary Vascular Cell Proliferation.

• DOI: 10.1161/circresaha.122.321836
• 发表时间: 2023-12-08
• 期刊: CIRCULATION RESEARCH
• 影响因子: 20.1
• 作者: Knight, Hannah;Abis, Giancarlo;Kaur, Manpreet;Green, Hannah L. H.;Krasemann, Susanne;Hartmann, Kristin;Lynham, Steven;Clark, James;Zhao, Lan;Ruppert, Clemens;Weiss, Astrid;Schermuly, Ralph T.;Eaton, Philip;Rudyk, Olena
• 通讯作者: Rudyk, Olena