Imaging Life with Light and Sound: State-of-the-art Photoacoustic Imaging For Mechanistic Understanding Of Human Disease

用光和声音成像生命：最先进的光声成像用于理解人类疾病的机制

• 批准号: MR/X012549/1
• 负责人: Maya Thanou
• 金额: $ 69.91万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX012549%2F1
• 关键词: Imaging; Life; Light; Sound; State

Photoacoustic imaging (PAI) involves shining a particular type of light into the body and then detecting sound waves coming out of the body. This can provide pictures showing the structure of organs, how they are working, and what certain molecules are doing in the body. PAI is safe and can look relatively deep into the body. It is important to be able to study how organs work in the body, what is happening in disease, in a living subject, rather than "in a dish" which does not reflect so well what happens in the body.We will use the most advanced PAI system commercially available to study heart, cardiovascular and brain disease and cancer in experimental models. PAI can be used to study organs such as the heart and the brain in living subjects, to see if organs are working properly, e,g., is the heart beating properly, is a blood vessel blocked, is blood flowing properly in the brain, is enough oxygen being delivered to the brain, etc. We can also use PAI to see if there is disease anywhere in the body, e,g., cancer. PAI can be used to see particular molecules that are changed when there is disease, e.g., the abnormal protein clumps in the brain in Alzheimer's and Parkinson's Disease. Sometimes molecules have to be tagged to be seen by PAI, and in this project, we will invent new tags/probes to see molecules we think are involved in causing disease. Disease can also be caused by certain cells not working properly and we can also tag these cells so that we can use PAI to see where they are and what they are doing. Sometimes drugs are delivered by specially made tiny particles or even in safe virus particles, and these can also be tagged so that we can see where drugs are being delivered by PAI. Using PAI we will get detailed pictures of what happens during human disease so that we can identify harmful molecules or cells that are important in causing disease and develop new drugs that will target these molecules and cells.

光声成像（PAI）涉及将特定类型的光照射到体内，然后检测从体内发出的声波。这可以提供显示器官结构、它们如何工作以及某些分子在体内做什么的图片。 PAI比较安全，可以比较深入地观察身体。重要的是能够研究器官在体内如何工作、疾病中发生的情况、活体中发生的情况，而不是“在培养皿中”，因为“在培养皿中”不能很好地反映体内发生的情况。我们将使用最多的先进的 PAI 系统可用于在实验模型中研究心脏、心血管和脑部疾病以及癌症。 PAI可用于研究活体受试者的心脏和大脑等器官，看看器官是否正常工作，例如心脏是否正常跳动、血管是否阻塞、大脑中的血液是否正常流动、是否有足够的氧气输送到大脑等。我们还可以使用PAI来查看身体任何部位是否有疾病，例如癌症。 PAI 可用于观察疾病发生时发生变化的特定分子，例如阿尔茨海默病和帕金森病大脑中的异常蛋白质团块。有时，分子必须被标记才能被 PAI 看到，在这个项目中，我们将发明新的标签/探针来查看我们认为与引起疾病有关的分子。疾病也可能是由某些细胞无法正常工作引起的，我们还可以标记这些细胞，以便我们可以使用 PAI 来查看它们在哪里以及它们在做什么。有时药物是通过特制的微小颗粒甚至安全的病毒颗粒来传递的，这些颗粒也可以被标记，以便我们可以看到 PAI 将药物传递到哪里。使用 PAI，我们将获得人类疾病期间发生的情况的详细图片，以便我们能够识别对引起疾病很重要的有害分子或细胞，并开​​发针对这些分子和细胞的新药物。

项目成果

Cyclin D-CDK4 Disulfide Bond Attenuates Pulmonary Vascular Cell Proliferation.

• DOI: 10.1161/circresaha.122.321836
• 发表时间: 2023-12-08
• 期刊: CIRCULATION RESEARCH
• 影响因子: 20.1
• 作者: Knight, Hannah;Abis, Giancarlo;Kaur, Manpreet;Green, Hannah L. H.;Krasemann, Susanne;Hartmann, Kristin;Lynham, Steven;Clark, James;Zhao, Lan;Ruppert, Clemens;Weiss, Astrid;Schermuly, Ralph T.;Eaton, Philip;Rudyk, Olena
• 通讯作者: Rudyk, Olena